Planta Med 2016; 82(01/02): 46-57
DOI: 10.1055/s-0035-1557829
Biological and Pharmacological Activitiy
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Pheophorbide a from Capsosiphon fulvescens Inhibits Advanced Glycation End Products Mediated Endothelial Dysfunction

Chung-Oui Hong*
1  Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, South Korea
,
Mi-Hyun Nam*
1  Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, South Korea
,
Ji-Sun Oh
1  Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, South Korea
,
Jin-Won Lee
2  Department of Food and Biotechnology, College of Science and Engineering, Hankyung National University, Anseong-si, Kyonggi-do, South Korea
,
Cheong-Tae Kim
3  Nong shim Co. 370, Shindaebang-dong, Dongjak-ku, Seoul, South Korea
,
Kwon-Woo Park
4  Division of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, South Korea
,
Dong-Ho Lee
4  Division of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, South Korea
,
Kwang-Won Lee
1  Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, South Korea
› Author Affiliations
Further Information

Publication History

received 30 November 2014
revised 22 June 2015

accepted 09 July 2015

Publication Date:
26 August 2015 (eFirst)

Abstract

During hyperglycemia, the first step toward the formation of advanced glycation end products is the nonenzymatic glycation between the carbonyl group of a sugar and the primary amino group of a protein. Advanced glycation end products are then produced through more complex reactions. Reactive oxygen species derived from advanced glycation end products may play a key role in inflammation of the endothelium, leading to the complications seen in diabetes. Glycolaldehyde-induced advanced glycation end products have been reported to express proinflammatory cytokines, such as tumor necrosis factor-α and interleukin-1β. This study focused on Capsosiphon fulvescens, a Capsosiphonaceae type of green algae that has shown potential as a functional food material. Pheophorbide a, an anti-glycation compound, was isolated from C. fulvescens by extraction using a mixture of ethanol and water, followed by column fractionation of the resulting extract. The compound separated from C. fulvescens was identified by means of high-performance liquid chromatography combined with mass spectrometry. Pheophorbide a showed scavenging activity of the intracellular reactive oxygen species as well as monocyte adhesiveness inhibitory activity on the human myelomonocytic cell line (THP-1) and human umbilical vein endothelial cells cocultivation system. The mRNA levels of inflammation-related genes such as monocyte chemoattractant protein-1 and interleukin-6 were significantly decreased by pheophorbide a, and advanced glycation end products-stimulated tumor necrosis factor-α and interleukin-1β were downregulated as well. These results indicate that pheophorbide a has significant reactive oxygen species-scavenging activity, monocyte adhesive inhibitory activity, and downregulatory activity of cytokines related to inflammation affecting the endothelium. Pheophorbide a could therefore be a promising candidate for modulating endothelial cell dysfunction.

* These two authors have contributed equally to this work and should be considered co-first authors.