Planta Medica Letters 2015; 2(01): e25-e27
DOI: 10.1055/s-0035-1557764
Letter
Georg Thieme Verlag KG Stuttgart · New York

Cytokine-Suppressive Activity of a Hydroxylated Alkylamide from Echinacea purpurea

Martha Leyte-Lugo
1  Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, NC, United States
,
Daniel A. Todd
1  Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, NC, United States
,
Travis V. Gulledge
2  Department of Biological Sciences, North Carolina State University, Raleigh, NC, United States
,
Monika Juzumaite
2  Department of Biological Sciences, North Carolina State University, Raleigh, NC, United States
,
Frederick S. Carter
1  Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, NC, United States
,
Scott M. Laster
2  Department of Biological Sciences, North Carolina State University, Raleigh, NC, United States
,
Nadja B. Cech
1  Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, NC, United States
› Author Affiliations
Further Information

Publication History

received 10 June 2015

accepted 23 June 2015

Publication Date:
04 August 2015 (online)

Abstract

Echinacea purpurea has been widely used to treat upper respiratory tract infections. Bioassay-guided fractionation of hexane and ethanol extracts of this plant yielded 4[(2-methylbutyl)amino]-4-oxo-2-butenoic acid (1) and 8,11-dihidroxy-dodeca-2E,4E,9E-trienoic acid isobutylamide (2), both of which are new to Echinacea. Compound 2 suppressed the production of TNF-α from RAW 264.7 cells with an IC50 value of 6.8 µM, while 1 was inactive. Neither compound showed cytotoxicity at concentrations up to 100 µM. These findings support a growing body of literature demonstrating that E. purpurea contains an array of compounds that suppress cytokine secretion by macrophage-type cells in vitro.

Supporting Information