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J Pediatr Infect Dis 2007; 02(03): 141-146
DOI: 10.1055/s-0035-1557042
Original Article
Georg Thieme Verlag KG Stuttgart – New York

Alterations in immunophenotype of peripheral blood mononuclear cells in children with chronic viral hepatitis following anti-viral therapy

Iwona Mozer-Lisewska
a   Department of Infectious Diseases and Child Neurology, University of Medical Sciences, Poznań, Poland
,
Grzegorz Dworacki
b   Department of Clinical Immunology, University of Medical Sciences, Poznań, Poland
,
Wojciech Służewski
a   Department of Infectious Diseases and Child Neurology, University of Medical Sciences, Poznań, Poland
,
Mariusz Kaczmarek
b   Department of Clinical Immunology, University of Medical Sciences, Poznań, Poland
,
Magdalena Figlerowicz
a   Department of Infectious Diseases and Child Neurology, University of Medical Sciences, Poznań, Poland
,
Arleta Kowala-Piaskowska
a   Department of Infectious Diseases and Child Neurology, University of Medical Sciences, Poznań, Poland
,
Anna Mania
a   Department of Infectious Diseases and Child Neurology, University of Medical Sciences, Poznań, Poland
,
Mirosław Szczepański
b   Department of Clinical Immunology, University of Medical Sciences, Poznań, Poland
,
Jan Żeromski
b   Department of Clinical Immunology, University of Medical Sciences, Poznań, Poland
› Author Affiliations
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Further Information

Publication History

19 October 2006

04 April 2007

Publication Date:
28 July 2015 (online)

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Abstract

The aim of this study was to investigate the impact interferon type I based anti-viral therapy on the immune system of chronically infected children with hepatitis C and B virus. Peripheral blood mononuclear cells were examined by three-color flow cytometry, using a panel of fluorochrome labeled monoclonal antibodies. It was found that the majority of white blood cell subsets were decreased in patients after termination of long-term anti-viral treatment. In particular, potentially cytotoxic, effector cell subsets, such as natural killer, natural killer T-lymphocytes and CD8+CD28- T cells were significantly decreased. Moreover, plasmacytoid dendritic cells were decreased, both in percentage and in absolute values. These data suggest that interferon-α based treatment, apart from its anti-viral effect, has also remarkable impact on patient's cell-mediated immunity.

Keywords

Cell immunophenotype - viral hepatitis - children