Planta Med 2015; 81 - PX76
DOI: 10.1055/s-0035-1556520

Antioxidant activity of Moringa oleifera isothiocyanates

P Rojas-Silva 1, 2, TB Tumer 1, 3, A Pulev 1, I Raskin 1, C Waterman 1
  • 1Plant Biology Department, Rutgers, The State University of New Jersey, New Brunswick, NJ-USA
  • 2Center for Translational Research, School of Medicine, Universidad De Las Américas UDLA, Quito, Ecuador
  • 3Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

Moringa oleifera Lam. (Brassicales) is an edible tropical tree with medicinal properties. Here, we describe a fractionation process of moringa leaves by fast centrifugal partition chromatography (FCPC) to generate polyphenol and isothiocyanate (ITC) rich fractions (Fig. 1). The polyphenol-rich fraction showed direct antioxidant activity assayed by oxygen radical absorbance capacity (ORAC), while the ITC-rich fraction demonstrated indirect antioxidant properties assayed by NQO1 activity in Hepa1c1c7 cells. Also, purified 4-[(α-l-rhamnosyloxy) benzyl] isothiocyanate (ITC-1), and 4-[(4'-O-acetyl-α-l-rhamnosyloxy)benzyl] isothio-cyanate (ITC-4) were evaluated for their ORAC and NQO1 inducer potency in comparison with sulforaphane (SF) at 5µM (Fig. 2). Both ITCs were as potent as SF in inducing NQO1 activity. These findings suggest that edible M. oleifera leaves contain a potent mixture of antioxidants that could explain its health promoting effects.