Development and validation of an RP-HPLC-PDA method for quantifying juglalin in Hydrangea seemannii phytopreparations

Hydrangea seemannii L. Riley (Hydrangeaceae) is a medicinal Mexican plant highly valued for the treatment of type II diabetes mellitus (TII-DM). Recently, we reported the antidiabetic action of an infusion of this plant and isolated the main active components, which included isoquercitrin, hyperoside, trifolin, guaijaverin, astragalin, and juglalin (kaempferol 3-O-α-L-arabinopyranoside). Hence, a suitable reversed-phase high-pressure liquid chromatography-photodiode array detection (RP-HPLC-PDA) method was developed for quantifying juglalin, the marker compound. The mobile phase consisted of a linear gradient of water (containing 0.1% formic acid) and acetonitrile (ACN) of 95:5 (0 min) to 100 of ACN (15 min) at a flow rate of 1.2 mL min^-1 and the detection was carried out at 254 and 327nm. The method was successfully validated in terms of linearity, accuracy, precision, and detection and quantitation limits. This procedure is appropriated for quality control and elaboration of standardized phytopreparations of H. seemannii.