INvolvement of caspase-dependent mitochondrial patyway in CORM-2 induced apoptosis in neuronal cells

Y Liu; J Wang

doi:10.1055/s-0035-1556432

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Planta Med 2015; 81 - PW4
DOI: 10.1055/s-0035-1556432

INvolvement of caspase-dependent mitochondrial patyway in CORM-2 induced apoptosis in neuronal cells

Y Liu ¹, J Wang ¹

¹Department of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China

Congress Abstract

The aim of this study is to investigate the effects of Tricarbonyldichlororuthenium (II) dimer (CORM-2) induced apoptosis and its mechanism in neuronal cells of rats. Optical microscope was applied to observe the morphologic changes of neuronal cells. MTT assay was performed to assess the survival rates of CORM-2 on neuronal cells. Apoptosis was examined by flow cytometric analysis and the expression of relative proteins was measured by Western Blotting analysis. The results suggested that CORM-2 could influence survival rates in a time- and concentration-dependent manner. Survival rates decreased gradually after the cultures subjected to 24h with 100µmol·L^-1, 200µmol·L^-1, 400µmol·L^-1 and 800µmol·L^-1 CORM-2. After 24h treatment, CORM-2 could induce neuronal apoptosis and activate Caspase-3, Caspase-9 and Cytochrome C, the main proteins in Caspase-dependent mitochondrial pathway in a concentration-dependent manner. To sum up, CORM-2 maybe induced neuronal apoptosis through Caspase-dependent mitochondrial pathway.