Planta Med 2015; 81 - PG13
DOI: 10.1055/s-0035-1556244

Biological activities of triterpenes isolated from the medicinal mushroom Ganoderma lucidum

D Sliva 1, 2, 3, J Loganathan 3, M Dudek 4, M Glensk 5, VB Glinski 5
  • 1DSTest Laboratories, Purdue Research Park, 5225 Exploration Dr, Indianapolis, IN 46241
  • 2Indiana University School of Medicine, Indianapolis, IN 46202, USA
  • 3Indiana University Health, Indianapolis, IN 46202
  • 4Medical University of Warsaw, Dept of Physical Chemistry, Warsaw, Poland
  • 5Planta Analytica, 39 Rose St, Danbury, CT 06810

Methanol extract of Ganoderma lucidum subjected to fractionation on silica gel medium pressure column afforded over 40 fractions. Further fractionation was carried out using Centrifugal Partition Chromatography (FCPC Rousselet Robatel, France), in the hexane-EtOAc-MeOH-Water systems adjusted for the polarity of each fraction. Some isolates were further purified by preparative HPLC. Methyl esters of ganoderic and lucidenic acids were obtained by methylation of the acids. Twelve compounds, including ganoderic acid A (1), methyl ganoderate A (2), methyl lucidenate A (3), methyl lucidenate F (4), lucidenic acid D2 (5), lucidenic acid A (6), lucidenic acid N (7), ganoderic acid DM (8), ganoderic acid F (9), ganoderic acid C (10), ganoderiol F (11) and ganodermonondiol (12) were evaluated for their biological activities. Antibacterial activity was determined against Gram -positive and -negative bacteria, anti-inflammatory effect against endotoxin (lipolysaccharide, LPS) induced inflammatory response in human macrophages, and anti-proliferative effects in human breast and colon cancer cells. We identified ganoderic acid DM as the most potent triterpene with anti-bacterial, anti-inflammatory and anti-proliferative effects when compared to other Ganoderma isolates.