Journal of Pediatric Epilepsy 2015; 04(02): 067-071
DOI: 10.1055/s-0035-1555599
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Significance of Glutathione-Mediated Scavenger Potency in the Development of Seizure Susceptibility in the EL Mouse Brain

Yasuhiko Kawakami
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
2   Department of Pediatrics, Nippon Medical School Tama Nagayama Hospital, Tama, Tokyo, Japan
,
Yoshiya L. Murashima
3   Graduate School of Human Health Science, Tokyo Metropolitan University, Tokyo, Japan
,
Mitsutoshi Tsukimoto
4   Department of Radiation Biology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan
,
Asami Baba
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
,
Chiharu Miyatake
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
,
Tetsuya Okazaki
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
,
Atsushi Takagi
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
,
Shinya Koizumi
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
,
Shuji Kojima
4   Department of Radiation Biology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan
,
Osamu Fujino
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
,
Yasuhiko Itoh
1   Department of Pediatrics, Nippon Medical School, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

12 May 2014

10 December 2014

Publication Date:
31 July 2015 (online)

Abstract

It has been reported that oxidative stress is closely related to the development of seizure susceptibility. To investigate if the redox condition shifts to an oxidized state during ictogenesis in epilepsy-prone EL mice, which shows the first seizure episode at 8 to 10 weeks of age, changes in the activities in the hippocampus or parietal cortex of reduced glutathione (GSH), oxidized glutathione, glutathione peroxidase, and nitric oxide were examined. This study showed that GSH activities were lower and oxidized glutathione activities were higher in EL mice than in ddY mice, mother strain control of EL, even though glutathione peroxidase activities were higher in EL mice between the ages of 3 and 8 weeks. Similarly, nitric oxide activities were higher in EL mice. The study also showed that GSH-mediated scavenger potency was lower not only in the hippocampus but also in the parietal cortex of EL mice at an early age. This reduction in GSH-mediated scavenger potency may be related to the acquisition of epileptogenesis in both the primary (parietal cortex) and secondary (hippocampus) epileptogenic centers of EL mice.