Planta Med 2015; 81(10): 791-797
DOI: 10.1055/s-0035-1546153
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Oxysophocarpine Ameliorates Carrageenan-induced Inflammatory Pain via Inhibiting Expressions of Prostaglandin E2 and Cytokines in Mice

Yang Yang*
1   Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, China
9   Department of Pharmacy, General Hospital of Ningxia medical University, Yinchuan, China
,
Yu-Xiang Li*
2   College of Nursing, Ningxia Medical University, Yinchuan, China
,
Hong-Ling Wang
1   Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Shao-Ju Jin
3   Luohe Medical College, Luohe, China
,
Ru Zhou
1   Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Hai-Qi Qiao
1   Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Juan Du
1   Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Jing Wu
4   Laboratory Animal Center, Ningxia Medical University, Yinchuan, China
,
Cheng-Jun Zhao
5   Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, China
,
Yang Niu
6   Key Laboratory of Hui Ethnic Medicine Modernization, Chinese Ministry of Education at Ningxia Medical University, Yinchuan, China
,
Tao Sun
7   Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, China
,
Jian-Qiang Yu
1   Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, China
8   Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine, Yinchuan, China
› Author Affiliations
Further Information

Publication History

received 27 February 2015
revised 29 April 2015

accepted 04 May 2015

Publication Date:
01 July 2015 (online)

Abstract

Oxysophocarpine is an alkaloid extracted from Sophora alopecuroides. We investigated the analgesic effect of oxysophocarpine on carrageenan-induced inflammatory pain in mice, in order to explore its possible mechanisms. Mouse ear swelling tests and carrageenan-induced paw edema tests were used to investigate the effects of oxysophocarpine on inflammatory pain in mice. Morphological changes on inflamed paw sections were measured by hematoxylin-eosin staining. The mRNA and protein expression of extracellular signal-regulated kinase, phosphorylation of extracellular signal-regulated kinase 1/2, cyclooxygenase-2, tumor necrosis factor α, interleukin-1 beta, interleukin-6 and prostaglandin E2 were investigated by real-time quantitative polymerase chain reaction, immunohistochemistry, western-blot and enzyme-linked immunosorbent assay. In our results, oxysophocarpine shows a significant anti-inflammatory effect in the mouse ear swelling test. Oxysophocarpine also significantly reduced the paw edema volume and improved mechanical allodynia threshold value on carrageenan-induced inflammatory pain, as well as relieved paw tissues inflammatory damage and reduced the numbers of neutrophils in mice. Oxysophocarpine significantly suppressed over-expression of cyclooxygenase-2, tumor necrosis factor α, interleukin-1 beta, interleukin-6 and prostaglandin E2, and inhibited the over-phosphorylation of extracellular signal-regulated kinase 1/2. Based on these findings we propose that oxysophocarpine attenuates inflammatory pain by suppressing the levels of phosphorylation of extracellular signal-regulated kinase 1/2, cyclooxygenase-2, prostaglandin E2, tumor necrosis factor α, interleukin-1 beta and interleukin-6.

* These authors contributed equally to this work.