Planta Medica Letters 2015; 2(1): e12-e14
DOI: 10.1055/s-0035-1545937
Letter
Georg Thieme Verlag KG Stuttgart · New York

Upregulatory Effects of Nobiletin, a Citrus Flavonoid with Anti-dementia Activity, on the Gene Expression of mAChR, ChAT, and CBP

Junko Kimura
1  Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
,
Kosuke Shimizu
1  Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
,
Jiro Takito
2  Department of Oral Anatomy and Developmental Biology, School of Dentistry, Showa University, Tokyo, Japan
,
Kiyomitsu Nemoto
3  Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
,
Masakuni Degawa
3  Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
,
Akihito Yokosuka
4  Laboratory of Medicinal Plant Science, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
,
Yoshihiro Mimaki
4  Laboratory of Medicinal Plant Science, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
,
Naoto Oku
1  Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
,
Yasushi Ohizumi
1  Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
5  Kansei Fukushi Research Institute, Tohoku Fukushi University, Sendai, Japan
› Author Affiliations
Further Information

Publication History

received 22 October 2014
revised 17 March 2015

accepted 20 March 2015

Publication Date:
23 April 2015 (online)

Abstract

We previously showed that nobiletin, a polymethoxylated flavone from the peels of Citrus depressa Hayata, improves impaired memory in dementia model animals. It was also reported that nobiletin stimulates cAMP response element-dependent transcription through activation of the cAMP response element-binding protein signaling pathway. To determine whether nobiletin alters the expression of genes involved in learning and memory, we performed real-time polymerase chain reaction after treating PC12 cells with nobiletin. Nobiletin upregulated the expression of muscarinic acetylcholine receptor subtype M1, choline acetyltransferase, and cAMP response element-binding protein genes in the cells. The muscarinic acetylcholine receptor M1 and choline acetyltransferase mRNA levels reached maximum values at 6 h after nobiletin treatment, whereas the cAMP response element-binding protein mRNA levels peaked at 12 h. These results suggest that nobiletin enhanced the expression of genes involved in learning and memory by stimulating the cAMP response element-dependent transcription.

Supporting Information