Planta Medica Letters 2015; 2(1): e15-e18
DOI: 10.1055/s-0035-1545936
Letter
Georg Thieme Verlag KG Stuttgart · New York

Neuroprotective Effect of Demethylsuberosin, a Proteasome Activator, against MPP+-induced Cell Death in Human Neuroblastoma SH-SY5Y Cells

Bo-Hyung Kim
1  Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Republic of Korea
,
Jaeyoung Kwon
2  Department of Biosystems and Biotechnology, Korea University, Seoul, Republic of Korea
,
Dongho Lee
2  Department of Biosystems and Biotechnology, Korea University, Seoul, Republic of Korea
,
Woongchon Mar
1  Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 21 December 2014
revised 13 March 2015

accepted 22 March 2015

Publication Date:
23 April 2015 (online)

Abstract

Demethylsuberosin isolated from the roots of Cudrania tricuspidata demonstrated a potent proteasome activator by enhancing all three chymotrypsin-like, trypsin-like, and caspase-like proteasome activities in a 20S proteasome activity assay. It also attenuated the 1-methyl-4-phenylpyridinium-induced dysfunction of the chymotrypsin-like and caspase-like activities of proteasome in SH-SY5Y cells with EC50 values of 0.76 µM and 0.82 µM, respectively. Additionally, demethylsuberosin protected neuronal cells against 1-methyl-4-phenylpyridinium-induced cell death with an EC50 value of 0.17 µM, while the EC50 value of betulinic acid was 4.29 µM. We are reporting that demethylsuberosin is a potent proteasome activator with a neuroprotective effect, suggesting a possible candidate for the protection or treatment of neurodegenerative diseases such as Parkinsonʼs disease.

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