Antitumor Properties of the Leaf Essential Oil of Zornia brasiliensis

Abstract

Zornia brasiliensis, popularly known as “urinária”, “urinana”, and “carrapicho”, is a medicinal plant used in Brazilian northeast folk medicine as a diuretic and against venereal diseases. The aim of this study was to investigate the chemical composition and antitumor potential of the leaf essential oil of Z. brasiliensis. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed by GC-MS and GC-FID. Its composition was characterized by the presence of trans-nerolidol, germacrene D, trans-caryophyllene, α-humulene, and farnesene as major constituents. In vitro cytotoxicity of the essential oil and some of its major constituents (trans-nerolidol, trans-caryophyllene, and α-humulene) was evaluated for tumor cell lines from different histotypes using the Alamar blue assay. The essential oil, but not the constituents tested, presented promising cytotoxicity. Furthermore, mice inoculated with B16-F10 mouse melanoma were used to confirm its in vivo effectiveness. An in vivo antitumor study showed tumor growth inhibition rates of 1.68–38.61 % (50 and 100 mg/kg, respectively). In conclusion, the leaf essential oil of Z. brasiliensis presents trans-nerolidol, germacrene D, trans-caryophyllene, α-humulene, and farnesene as major constituents and is able to inhibit cell proliferation in cultures as well as in tumor growth in mice.

Key words

Zornia brasiliensis - Fabaceae - essential oil - cytotoxicity - B16-F10

• References

• 1 Mohlenbrock RJ. A monograph of the leguminous genus Zornia . Webbia 1961; 16: 1-141
• 2 McIvor JG, Bray RA. Genetic resources of forage plants. Melbourne: CSIRO; 1983
• 3 Kloos H, Thiongo FW, Ouma JH, Butterworth AE. Preliminary evaluation of some wild and cultivated plants for snail control in Machakos District, Kenya. J Trop Med Hyg 1987; 90: 197-204
• 4 Rojas A, Bah M, Rojas JI, Serrano V, Pacheco S. Spasmolytic activity of some plants used by the Otomi Indians of Quéretaro (México) for the treatment of gastrointestinal disorders. Phytomedicine 1999; 6: 367-371
• 5 Obi CL, Ramalivhana J, Samie A, Igumbor EO. Prevalence, pathogenesis, antibiotic susceptibility profiles, and in-vitro activity of selected medicinal plants against Aeromonas isolates from stool samples of patients in the Venda region of South Africa. J Health Popul Nutr 2007; 25: 428-435
• 6 David JP, Meira M, David JM, Brandão HN, Branco A, de Fátima Agra M, Barbosa MR, de Queiroz LP, Giulietti AM. Radical scavenging, antioxidant and cytotoxic activity of Brazilian Caatinga plants. Fitoterapia 2007; 78: 215-218
• 7 Arunkumar R, Nair SA, Subramoniam A. Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers. J Pharmacol Pharmacother 2012; 3: 233-241
• 8 Silva AD, Cavalcante-Silva LH, da Matta CB, Silva Dde F, Araújo MV, Tavares JF, da Silva MS, Alexandre-Moreira MS. Antinociceptive effect of 7-methoxyflavone isolated from Zornia brasiliensis . Nat Prod Res 2013; 27: 1695-1699
• 9 Agra MF, Silva KN, Basílio IJLD, Freitas PF, Barbosa-Filho JM. Survey of medicinal plants used in the region Northeast of Brazil. Braz J Pharmacogn 2008; 18: 472-508
• 10 Arunkumar R, Nair SA, Rameshkumar KB, Subramoniam A. The essential oil constituents of Zornia diphylla (L.) Pers, and anti-inflammatory and antimicrobial activities of the oil. Rec Nat Prod 2014; 8: 385-393
• 11 Britto AC, Oliveira AC, Henriques RM, Cardoso GM, Bomfim DS, Carvalho AA, Moraes MO, Pessoa C, Pinheiro ML, Costa EV, Bezerra DP. In vitro and in vivo antitumor effects of the essential oil from the leaves of Guatteria friesiana . Planta Med 2012; 78: 409-414
• 12 Ferraz RP, Cardoso GM, da Silva TB, Fontes JE, Prata AP, Carvalho AA, Moraes MO, Pessoa C, Costa EV, Bezerra DP. Antitumour properties of the leaf essential oil of Xylopia frutescens Aubl. (Annonaceae). Food Chem 2013; 141: 196-200
• 13 Ribeiro SS, Jesus AM, Anjos CS, Silva TB, Santos AD, Jesus JR, Andrade MS, Sampaio TS, Gomes WF, Alves PB, Carvalho AA, Pessoa C, de Moraes MO, Pinheiro ML, Prata AP, Blank AF, Silva-Mann R, Moraes VR, Costa EV, Nogueira PC, Bezerra DP. Evaluation of the cytotoxic activity of some Brazilian medicinal plants. Planta Med 2012; 78: 1601-1606
• 14 Fontes JEN, Ferraz RP, Britto AC, Carvalho AA, Moraes MO, Pessoa C, Costa EV, Bezerra DP. Antitumor effect of the essential oil from leaves of Guatteria pogonopus (Annonaceae). Chem Biodivers 2013; 10: 722-729
• 15 Tundis R, Loizzo MR, Bonesi M, Menichini F, Dodaro D, Passalacqua NG, Statti G, Menichini F. In vitro cytotoxic effects of Senecio stabianus Lacaita (Asteraceae) on human cancer cell lines. Nat Prod Res 2009; 23: 1707-1718
• 16 Silva EB, Matsuo AL, Figueiredo CR, Chaves MH, Sartorelli P, Lago JH. Chemical constituents and cytotoxic evaluation of essential oils from leaves of Porcelia macrocarpa (Annonaceae). Nat Prod Commun 2013; 8: 277-279
• 17 Gautam N, Mantha AK, Mittal S. Essential oils and their constituents as anticancer agents: a mechanistic view. Biomed Res Int 2014; 2014: 154106
• 18 Seo WH, Baek HH. Identification of characteristic aroma-active compounds from water dropword (Oenanthe javanica DC.). J Agric Food Chem 2005; 53: 6766-6770
• 19 Dugo P, Mondello L, Zappia G, Bonaccorsi I, Cotroneo A, Russo MT. The composition of the volatile fraction and the enantiomeric distribution of five volatile components of faustrime oil (Monocitrus australatica x Fortunella sp. x Citrus aurantifolia). J Essent Oil Res 2004; 16: 328-333
• 20 Fidler I. Selection of successive tumor lines for metastasis. Nat New Biol 1973; 242: 148-149
• 21 Van Den Dool H, Kratz PD. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J Chromatogr 1963; 11: 463-471
• 22 Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. Illinois: Allured Publishing Corporation; 2007
• 23 Ahmed SA, Gogal RM, Walsh JE. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes an alternative to [³H] thymidine incorporation assay. J Immunol Methods 1994; 170: 211-224