Quantification of naringenin encapsulated in solid lipid nanoparticles by validated UV-spectroscopy method

Naringenin is a citrus flavonoid mainly obtained from citrus fruits and tomato. It possesses various promising therapeutic effects in-vitro and in-vivo. The purpose of this research work was to develop and validate a simple, specific, rapid, precise and highly accurate UV-spectrophotometric method for quantification of naringenin from solid lipid nanoparticles. This method was based on the measurement of absorption of UV light. Validation of the analytical method was performed as per ICH Q2 (R1) guidelines which require investigating parameters like linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. The absorption maximum (λ_max) of naringenin in methanol was fund to be 283.49nm. The linearity for naringenin was in the range of 0 – 25 µg/mL with high correlation coefficient value (R²= 0.9996). The intraday and interday precision was determined with RSD of 0.38%, 0.11 – 0.39% and 0.08 – 0.39%, respectively. The percentage recoveries of naringenin were found to be 98.39 – 102.55% with RSD 0.38% implied high accuracy of the method. Limit of detection and limit of quantitation were found to be 0.1137 and 0.3448 µg/ml, respectively suggesting good sensitivity of the method. The RSD value of robustness studies were found to be less than 2%, which indicates that the method was robust. The method is envisaged to be effectively used for routine quality control assay for encapsulated naringenin in solid lipid nanoparticles.