Planta Med 2015; 81 - PB12
DOI: 10.1055/s-0035-1545167

In Vitro Propagation of Cannabis sativa L. Using meta-Topolin

H Lata 1, S Chandra 1, IA Khan 1, 2, MA ElSohly 1, 3
  • 1National Center for Natural Product Research, Research Institute of Pharmaceutical Sciences
  • 2Department of BioMolecular Sciences
  • 3Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS 38677, USA

Cannabis sativa is one of the oldest known domesticated plants belonging to the family Cannabaceae. It is extensively cultivated throughout the world for its psychoactive properties, durable fiber and seeds. The plant is reported to have several medicinal properties. The present study describes a simple, efficient and one step regeneration system for rapid axillary shoot proliferation and in vitro rooting of C. sativa nodal explants using meta-Topolin (mT), an aromatic natural cytokinin. The highest shoot proliferation frequency was obtained from nodal explants cultured on Murashige and Skoog (MS) basal salts supplemented with 0.5 mg l–1 meta-topolin (mT) generating an optimal number of shoots with suitable morphological features. Shoots multiplied on the same medium for two subcultures of three weeks each, were able to induce healthy roots. Regenerated plantlets were successfully acclimatized and hardened off inside the culture room and then transferred to a greenhouse with 100% survival rate.