Planta Medica Letters 2015; 2(1): e1-e5
DOI: 10.1055/s-0034-1396321
Letter
Georg Thieme Verlag KG Stuttgart · New York

Mycosporine-Like Compounds in Chlorolichens: Isolation from Dermatocarpon luridum and Dermatocarpon miniatum, and their Photoprotective Properties

Thi Thu Tram Nguyen
Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
,
Marylène Chollet-Krugler
Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
,
Françoise Lohézic-Le Dévéhat
Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
,
Isabelle Rouaud
Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
,
Joël Boustie
Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
› Author Affiliations
Further Information

Publication History

received 26 July 2014
revised 26 December 2014

accepted 15 January 2015

Publication Date:
03 March 2015 (online)

Abstract

Two mycosporines were isolated for the first time in two chlorolichen species, Dermatocarpon luridum and Dermatocarpon miniatum. Mycosporine glutaminol (1) and mycosporine glutamicol (2) were isolated along with the ethyl ester of mycosporine glutamicol (3), which was formed during the purification process. Aqueous extracts and pure mycosporines were then investigated for their antioxidant activities and photoprotective properties along with their photostability and photocytotoxicity. Semi-purified mycosporine fractions were much more antioxidant than lichen aqueous crude extracts. Compound 3 (IC50 = 4.00 µg/mL) was found to be as active as quercetin (IC50 = 6.75 µg/mL), while the two genuine mycosporines 1 and 2 exhibited moderate activity. The three mycosporines were found to be stable until 5J/m2 UVA and UVB radiations whereas Trolox, used as a positive control, was degraded up to 10 % and 19 %, respectively. Moreover, these mycosporines and semi-purified extracts did not induce any phototoxicity on HaCaT cells exposed to UVA radiations.

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