In vitro trail for stimulating callus growth and its ingredient content of Zingiber officinale plant
Different explants of Zingiber officinale were in vitro cultured in MS medium containing of various levels and types of growth regulators. The results shows that concentrations of 0.5 mg/l. BA +1.0 mg/l. 2,4-D recorded the highest significant value of callus formation from shoot tip. For callus growth concentrations of (1.0 mg/l. BA + 0.5 mg/l. 2,4-D) and (1.0 mg/l. BA + 1.0 mg/l. 2,4-D) caused the highest significant value of callus growth. On the other hand, the callus produced from previous experiment was supplemented to MS medium containing (1.0 mg/l. BA + 0.5 mg/l. 2,4-D) employed to study the influence of precursors (mevalonic acid, phenylalanine, valine, and leucine) and biotic elicitors (yeast extract and Aspergillus niger) on the callus growth and its ingredient contents. 6-gengerol production (30.0 µg/100 mg F.W.) accumulation was obtained in callus treated with 10 mg/l. of mevalonic acid, followed by that treated with 250 mg/l. yeast extract (10.0 µg/100 mg F.W. of 6-gengerol) while using 250 mg/l. A. niger gave the lowest value of 6-gengerol (6.0 µg/100 mg F.W.).