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Antimicrobial activity of extracts of leaves of Annona hypoglauca
The aim of this study was to investigate the antimicrobial activity from hexane (EH), chloroform (CE) and ethanol (EE) extracts from the leaves of Annona hypoglauca collected in the Mucajaí city, Roraima, Brazil, by the method of Minimum Inhibitory Concentration (MIC). A voucher (UFRR 3653) was deposited in the Herbarium of the Federal University of Roraima and registered in the Biodiversity Information System – SISBIO (416227 – 1), Ministry of Environment of Brazil. The concentrations evaluated ranged from 3.90 mg/mL to 500 mg/mL and the extracts were tested against fungi (Aspergillus flavus and Candida albicans) and bacteria (Staphylococcus aureus, Streptococcus sanguinis, Escherichia coli and Salmonella typhimurium). The MIC of EH was 500 mg/mL against S. aureus, with 100% inhibition, while the EC presented 66% of effectiveness at the same concentration. EE presented no significant activity against this bacterium. There was 100% inhibition of S. sanguinis at 500 mg/mL by EH. For E. coli no significant activity was detected for none of the extracts since inhibition was lower than 50% inhibition for all tested doses. EE presented 61% inhibition of S. typhimurium at 250 mg/mL and CE presented 58% inhibition at a low doses (9.375 mg/mL). It was observed excellent activity against C. albicans even at the lowest concentrations. Towards A. flavus only EH was active (59% inhibition) at 250 mg/mL. The control used for bacteria was Ampicillin and for fungi Nystatin and Miconazole were used. Brazil has 33 of Annona native genus with about 250 species . There is a study of the bioactive compounds of the timber A. hypoglauca against tumor cells, but no studies of the leaves of the species . Several studies show antimicrobial potential of the Annona genus [3, 4], but for the species under study no reports of antimicrobial activity of its leaves were found.
Keywords: Antimicrobial, vegetable extracts, Annonaceae
 Souza, V.C., et al. (2008) Botânica sistemática: guia ilustrado para identificação das famílias de fanerógamas nativas e exóticas do Brasil, baseado APGII. 2ed. Nova Odessa, SP: Instituto Plantarum.
 Rinaldi, M.V. (2007) Avaliação da atividade antibacteriana e citotóxica dos alcaloides isoquinolínicos de Annona hypoglauca Mart. [Dissertação] Universidade de São Paulo.
 El-Chaghaby G.A., et al. (2014) Evaluation of the antioxidant and antibacterial properties of various solvents extracts of Annona squamosa L. leaves. Arab. J. Chemistry, 7: 227 – 233.
 Padmaja V., et al. (1995) Biological activities of Annona glabra. J. Ethnopharm., 48: 21 – 24.