Planta Med 2014; 80 - P2O73
DOI: 10.1055/s-0034-1395063

The inhibitory effect of chemical constituents derived from Artocarpus xanthocarpus on melanin biosynthesis

HH Ko 1, YJ Jin 1, CC Lin 2, TM Lu 3, JH Li 2, IS Chen 2, 4
  • 1Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
  • 2School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
  • 3Department of Pharmacy, Tajen University, Pingtung County 90741, Taiwan
  • 4Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

Eighteen known compounds and six new compounds were isolated from the roots of Artocarpus xanthocarpus, and their structures were spectroscopically determined. The new compounds included artoxanthocarpuone A (1), artoxanthocarpuone B (2), hydroxylakoochin A (3), methoxylakoochin A (4), epoxylakoochin A (5), and artoxanthol (6). Among the isolated compounds, artoxanthol (6), arboctalol (9) steppogenin (11), norartocarpetin (12), resveratrol (19), oxyresveratrol (20), and chlorophorin (21) strongly inhibited mushroom tyrosinase activity with IC50 values of 5.7 ± 0.3, 6.4 ± 0.3, 1.9 ± 0.1, 0.9 ± 0.1, 4.9 ± 0.3, 1.0 ± 0.5, and 2.5 ± 0.4µM, respectively. Artoxanthocarpuone A (1), artoxanthocarpuone B (2), methoxylakoochin A (4), lakoochin A (8), cudraflavone C (17), artonin A (18), resveratrol (19), and chlorophorin (21) reduced tyrosinase activity and inhibited α-melanocyte-stimulating hormone-induced melanin production in B16F10 melanoma cells and were not cytotoxic. Collectively, our results suggest the potential utility of A. xanthocarpus-derived compounds as depigmenting agents.

Keywords: Constituents, Artocarpus xanthocarpus, tyrosinase, melanin