Planta Med 2014; 80 - P2O36
DOI: 10.1055/s-0034-1395028

HPLC fingerprint of Potentilla reptans L. (Rosaceae)

S Pilipovic 1, B Pilipovic 2, A Uzunovic 1, A Elezovic 1, A Boric 1, Z Ademovic 3
  • 1Agency for Medicinal Products and Medical Devices, Titova 9, 71000, Sarajevo, Bosnia and Herzegovina
  • 2Bosnalijek d.d., Jukiceva 53, 71000, Sarajevo, Bosnia and Herzegovina
  • 3Faculty of Pharmacy, Univerzitetska 9, 75000, Tuzla, Bosnia and Herzegovina

Most species of order Potentilla contain tannin [1] as active principle. Due to extinguished medical function many of them are used in traditional medicine for treatment of inner and outer fevers, against diarrhea, hart diseases or for heeling of ponderous wounds [2]. As sample we used rhizome collected on central Bosnia and Herzegovina. The examined material was picked up in July 2013, dried in thin layer and pulverized immediately before the experiment. Our goal was to made quick HPLC fingerprint method for detection of different catechins without numerous standards. We used a Shimadzu Class-VP HPLC with Class-VP 5.03 software and SCL-10A VP system controller, a Shimadzu DGU 14A Degasser, FCV-10AL Mixer, LC-10AD Shimadzu Liquid Chromatography Pumps, SIL-10a XL Auto Injector, a CTO-10A Column Oven, SPD-M10A VP Diode Array Detector and fluorescent detector RF-10AXL, column LiChrosorb RP-18 (250 × 4 mm I.D., 5 µm). Condition of chromatography was: owen temperature 35 °C, mobile phase A: 2% aqueous acetic acid solution, mobile phase B: acetonitrile 100%, with gradient from 8% till 31% B from beginning till 50 minutes, after that 100% B 2 min, and 100% B next 3 min, and again 8% B next 10 min (flow 1.2 ml/min). The injection volume of sample was 20µL. Sample for analysis was made from 4 g of dry pulvered rhizome with 180 ml of boiling water, and after 4 minutes in ultrasound mixer, we filtered extract trough filter 0,2 µm, cooled on room temperature and analyzed. As standard we used standard of catechin, and phenolic acids purchased from Sigma Aldrich. In obtained chromatographic we detected catechin, dimers of catechins (fluorescent respond excitation 283nm, adsorption on 317nm). Because of lack of standards we concluded that this method can be used as fingerprint of different sorts of Potentilla species.

Keywords: Potentilla reptans, HPLC fingerprint

References:

[1] Tomczyk M, Latte KP. Potentilla – A review of its phytochemical and pharmacological profile. J Ethnopharmacol. 2009; 122: 184 – 204.

[2] Redzic SS. The Ecological Aspect of Ethnobotany and Ethnopharmacology of Population in Bosnia and Herzegovina. Coll Antropol. 2007; 31: 869 – 890.