Investigation of Sida spinosa for antidiabetic potential in experimental induced diabetes in animals

Oxidative stress is known to be a component of molecular and cellular tissue damage mechanisms in a wide spectrum of human diseases. Excessive oxidative stress has been implicated in the pathology of Diabetes. Literature survey reveals that the role of Sida spinosa in terms of its antihyperglycemic, antioxidant evaluation remains unexplored. The present study is an attempt to explore and understand the underlying mechanism of active constituents present in S. spinosa and in management of diabetes and its antioxidant potential. Cold maceration and successive extraction was carried out using different solvent viz hexane, chloroform, ethyl acetate, butanol, aqueous. Diabetes was induced in male Wistar rats. Diabetic animals were treated with different solvent extracts of S. spinosa, Glipizide was used a standard drug. Blood Glucose level was determined. Blood plasma glucose levels of test and standard group animals were compared with diabetic control group at 1, 3 and 6 hr after respective treatment to study the single dose effect of drug on blood glucose levels. Test extract at 100 mg/kg did not show any significant effect and blood plasma glucose levels were not significantly reduced as compared to diabetic control. However, test extract at 200 mg/kg showed delayed response and significantly reduced blood glucose levels as compared to diabetic control at 6hr with p < 0.001 when tested for significance at 95% CI using One-Way ANOVA by Dunnett's test with diabetic control. Standard drug (Glipizide at 5 mg/kg) showed significant reduction in blood glucose when compared to diabetic control group at 1hr (p < 0.05) and at 3hr with p < 0.001 when tested for significance at 95% CI using One-Way ANOVA by Dunnett's test

Keywords: Sida spinosa