HS-SPME-GC-MS analysis of volatile and semi-volatile compounds from Mikania glomerata dried leaves

This study reports on the identification of volatile and semi-volatile compounds and a comparison of the chromatographic profiles obtained by HS-SPME-GC-MS (Headspace Solid Phase Microextraction – Gas Chromatography with Mass Spectrometry detection), from dried leaves of Mikania glomerata Sprengel (Asteraceae), one of the species known in Brazil as “guaco” and employed as raw material in phytopharmaceutical industry. Three different types of commercial SPME fibers were tested: poly(dimethylsiloxane) (PDMS), poly(dimethylsiloxane)-divinylbenzene (PDMS-DVB) and polyacrylate (PA). 59 compounds were fully identified by HS-SPME-HRGC-MS, most of them identified as being mono- and sesquiterpenes. Considering the sum of the areas of the detected peaks in TIC as a criterion to measure extraction efficiency, PDMS-DVB showed the highest efficiency. PA fiber showed better performance in the analysis of coumarin, a marker for the quality control of guaco-based phytomedicines [1]. Coumarin was better extracted by PA fiber than by conventional hydrodistillation of essential oils (EO), and the HS-SPME method also offers the advantages of requiring smaller samples and shorter time of analysis than the HPLC-UV and the HRGC-FID methods previously reported for M. glomerata analysis [1, 2]. The results enable us to suggest the use of HS-SPME-HRGC-MS method as a valuable tool for quality control of guaco-based phytomedicines and also for the screening and characterizing the chemical composition of volatile and semi-volatile compounds of M. glomerata in further studies requiring a large number of plant samples and/or when a limited amount of plant material is available for several samplings, such as metabolomic or agronomical studies.

Acknowledgements: FAPESP, CNPq

Keywords: Mikania glomerata Sprengel (Asteraceae), volatile compounds, coumarin, terpenoids, phytomedicines, HS-SPME-HRGC-MS

References:

[1] Celeghini RMS et al. Extraction and quantitative HPLC analysis of coumarin in hydroalcoholic extracts of Mikania glomerata Spreng. (“guaco”) leaves. J. Braz. Chem. Soc. 2001; 12: 706 – 709.

[2] Vilegas JHY et al. Determination of coumarin and kaurenoic acid in Mikania glomerata (“guaco”) leaves by capillary gas chromatography. Phytochem Anal 1997, 8: 74 – 77.