Planta Med 2014; 80 - P2B52
DOI: 10.1055/s-0034-1394929

Anti-inflammatory effect of Lippia origanoides

GM Figueiredo 1, CC Guilhon 1, SG Leitão 2, DR Oliveira 2, FCM Chaves 3, PD Fernandes 1
  • 1Federal University of Rio de Janeiro: Institute of Biomedical Science, Rio de Janeiro, Brazil
  • 2Department of Natural Products and Food, Faculty of Pharmacy, Rio de Janeiro, Brazil
  • 3Embrapa Amazônia Ocidental, Km 29, AM 010, Manaus, Brazil

Introduction: Lippia origanoides Kunth is an aromatic shrub known as “salva-do-marajó”. Aerial parts are used to treat gastrointestinal and respiratory problems and by Oriximiná (PA, Brazil) midwife to treat postpartum bleeding (“to clean woman inside”) or to woman inflammation [1]. Although there are ethnobotanical data, there is no scientific evidence of information obtained. In this regard this work aimed to evaluate the effects of ethanol extract from leaves of L. origanoides in models of inflammation.

Methods: L. origanoides was collected at Embrapa Amazônia Ocidental. Voucher (CESJ39532) is deposited at herbarium of Federal University of Juiz de Fora, Brazil. Leaves were dried, triturated and extracted by maceration with ethanol (EE). Webster mice (20 – 25 g, n = 6 – 8) were orally treated with EE (10, 30,100 mg/kg). Anti-inflammatory effect was evaluated by formalin [2] or subcutaneous air pouch (SAP) [3]. Results are presented as medium ± DP. Statistics was done by ANOVA/Bonferroni (*p < 0.05). Protocols for animal use number is #ICBDFBC-015 (COBEA/UFRJ/Brazil).

Results:

Formalin

Vehicle

10 mg/kg

30 mg/kg

100 mg/kg

1st phase
(seconds)

72.6 ± 18.6

67 ± 18.8
(7.7% reduction)

63.6 ± 17.5
(12.4% reduction)

39.4 ± 6.2*
(45.7% reduction)

2nd phase
(seconds)

236.1 ± 34.5

146.7 ± 46.4*
(37.9% reduction)

89.9 ± 25.4*
(61.9% reduction)

97.5 ± 20.6*
(58,7% reduction)

SAP

Vehicle

10 mg/kg

30 mg/kg

100 mg/kg

Leukocyte
migration
(cellx206/mL)

60.6 ± 5.5

37.3 ± 8.2*
(38.4% reduction)

34.6 ± 9.4*
(42.9% reduction)

33.4 ± 7.7*
(44.9% reduction)

TNF-α production
(pg/mL)

0.53 ± 0.1

0.2 ± 0.1*
(62.3% reduction)

0.2 ± 0.1*
(62.3% reduction)

0.2 ± 0.2*
(62.3% reduction)

Conclusion: Ethanol extract from leaves of L. origanoides has a significant anti-inflammatory effect confirming the traditional use.

Acknowledgements: Alan Minho (technical support), Instituto Vital Brazil (donation of animals), CAPES, CNPq and FAPERJ (financial support).

Keywords: Lippia origanoides, anti-inflammatory effect

References:

[1] Oliveira et al. 2014. Rev Bras Farmacog; 24: in press.

[2] Tsølsen A et al. 1992. Pain; 51: 5 – 17.

[3] Raymundo LJRP et al. 2001. J Ethnopharmacol; 134: 725 – 732.