Planta Med 2014; 80 - P2B50
DOI: 10.1055/s-0034-1394927

Lippia origanoides Kunth (Verbenaceae) in vitro culture: Volatile fraction composition and morphogenesis evaluation

N Silva 1, S Guimarães-Leitão 1, C de Oliveira-Miranda 1 C Castilho 1, et al
  • 1DPNA – Faculdade de Farmácia – Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, 21941 – 590, Rio de Janeiro, Brazil
  • 2Embrapa Agroindústria de Alimentos – Rio de Janeiro, Brazil

Lippia origanoides is a small shrub native from Brazil that is used for food and medicinal purposes [1]. Its essential oil (EO) is rich in thymol, carvacrol, and γ-terpinene. The specie grows only in wild habitats representing a risk for its conservation its large scale use. The aim of this work was to evaluate the effects of different plant growth regulators on L. origanoides in vitro development and volatile fraction (VF) composition. Single nodes were cultured on MS medium [2] supplemented with BA, KIN or IAA in different concentrations (0, 0.1, 0.5, 1 and 2 mgL-1). After 60 days, plants were evaluated for shoot number, shoot length (cm), fresh and dry weight and VF profile. The VF was obtained by SDE (2 g each treatment; 3h), and the VF was collected in CH2Cl2. The identification of the sample components was performed by GC-FID and GC-MS and the compounds were identified by comparison of their linear retention indices with Spectra library and published data [3]. The highest average shoot length was observed in plants from MS+0.5 mgL-1 KIN (3.15 cm), MS+1 mgL-1 KIN (4.10 cm), and MS+0.5 mgL-1 IAA (3.57 cm). The highest shoot number was obtained from MS without growth regulators (9.67), not statistically different from MS+2 mgL-1 BAP (8.37) and MS+1 mgL-1 KIN (8.0) media. Volatile compounds found in plants from MS and MS+2 mgL-1 BA were, respectively: carvacrol (40.2 and 39.1); p-cymene (10.2 and 11.4); γ-terpinene (6.8 and 4.8); thymol (5.5 and 6.3); β-caryophyllene (3.5 and 3.9); linalool (3.5 and 2.5); myrcene (2.4 and 2.4); thymol methyl ether (1.4 and 2.1); α-terpinene (1.5 and 1.3) and α-tujen (1.3 and 1.2). In conclusion, the chromatography analysis showed that all in vitro culture plants have similar VF profile and MS+1 mgL-1 KIN medium should be used for micropropagation.

Keywords: Lippia origanoides, micropropagation, cytokinin, auxin, thymol, carvacrol

References:

[1] Oliveira, D.R.; Leitão, G.G. et al. Food Chemistry, 2007, 101: 236 – 240.

[2] Murashige, T.; Skoog, F. Physiologia Plantarum, 1962, 15: 473 – 497.

[3] Adams, R.P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th ed. Carol Stream, IL: Allured Publishg Co. 2007. 804 p.