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DOI: 10.1055/s-0034-1394901
Wound healing effect of Tibouchina granulosa aqueous extract in diabetic animals
Introduction: Tibouchina granulosa is an ornamental species from Brazil, popularly known as “quaresmeira”. Leaves are traditionally used for the treatment of cutaneous wounds. However, there is no data of its pharmacological confirmation. In this work we evaluate the wound healing effect of leaves infusion of T. granulosa in diabetic mice as well as assayed the infusion for the presence of anthocyanins and tannins.
Methods: Leaves from quaresmeira were collected at Cachoeiras de Macacú (RJ/Brazil). Voucher (37,931) is deposited at herbarium of Biology Institute/UFRJ. Plant material was dried, triturated and an infusion was prepared (10% w/v). Webster diabetic mice (DM, 30 – 35 g, n = 10 – 15) were used in the cutaneous lesions model [1].They were locally treated with infusion(30µL). At days 0,7,10,14 wounds were photographed and images processed in ImageJ program to calculate the wound area. Preliminary phytochemical analyses were done by colorimetric assays/HPLC. Results are presented as medium ± DP of wound area (in arbitrary units). Statistic done by ANOVA/Bonferroni(*p < 0.05). Protocols for animal use number:ICBDFBC-015(COBEA/UFRJ/Brazil).
Results: Results (in AU of wound areas) are:
Group/Day |
0 |
7 |
10 |
14 |
Non-DM |
71.5 ± 12.4 |
21.2 ± 11 |
9 ± 6.8 |
1.2 ± 1.8 |
DM |
46.2 ± 10.5 |
36.4 ± 8.9 |
23 ± 12 |
4.4 ± 5.4* |
DM/infusion |
63 ± 19 |
31 ± 8.4* |
8 ± 2.9* |
2.4 ± 3.9* |
Conclusion: Infusion of T. granulosa significantly demonstrated a wound healing effect. This effect could be related, at least in part, to the presence of tannins/anthocyanins in the infusion (identified by HPLC/colorimetric assays). However, it is necessary to isolate the substances, identify then and test each one separately.
Acknowledgements: Alan Minho (Technical support), Instituto Vital Brazil (donation of animals), CNPq and FAPERJ (financial support).
Keywords: Tibouchina granulosa, wound healing, inflammation
References:
[1] Wong et al. 2011. J Biomed Biotechnol; 969618