Planta Med 2014; 80 - P2B23
DOI: 10.1055/s-0034-1394900

Evaluation of antinociceptive and/or anti-inflammatory activity of Choisya Aztec Pearl

PR Carvalho 1, CC Guilhon 1, D Ropero 2, F Boylan 2, PD Fernandes 1
  • 1Federal University of Rio de Janeiro, Institute of Biomedical Science, Laboratory of Pharmacology of Pain and Inflammation. Av. Carlos Chagas Filho 373, J10. Rio de Janeiro, Brazil
  • 2School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland

Introduction: C. Aztec Pearl (Rutaceae) is known as “Mexican orange”. It is a hybrid of C. Ternata and C. Dumosa var. arizonica. The traditional medicine C. Ternata has been used in Mexico, which infusions from the leaves claimed to have some effects such as antispasmodic and stimulant properties [1]. Our objective was to investigate a possible anti-inflammatory activity from extracts and fractions from C. Aztec Pearl's leaves.

Method: Leaves of C. Aztec were collected in Dublin, voucher name TCD Hodkinson & Ropero 01 deposited at TCD Herbarium. The ethanol extract (E) was submitted to a liquid-liquid extraction obtaining the fractions: hexane (H), ethyl acetate (EA), butanol (B) and residue (R). Antinociceptive and anti-inflammatory effects were evaluated by the model formalin-induced licking [2]. Mice (22 – 25 g, n = 4 – 6) were treated with 10, 30, 100 mg/kg. Results are presented as mean ± SD and statistical analysis were performed by ANOVA followed by Bonferroni test (*p < 0.05). Protocols for animal use number #ICBDFBC-015(COBEA/UFRJ/Brazil).Results: Although none of extracts inhibited the 1st phase, the majority of them reduced the 2nd phase, as shown on the table below.

Conclusions: The ethanol extract and H, EA, B and R fractions presented anti-inflammatory activity since significantly and dose dependently reduced the 2nd phase of the model.

Dose (mg/mg)

10

30

100

Vehicle 212 ± 21.6 s

E

74.6 ± 17.9*s

31.9 ± 49.3* s

69.8 ± 29.5* s

H

81.9 ± 10.1*s

31.1 ± 9.8*s

13.2 ± 8.6*s

EA

123.1 ± 15.5*s

68.4 ± 39.2* s

44 ± 13*s

B

145.1 ± 22.6 s

92.2 ± 18.8*s

102.9 ± 39.8*s

R

74 ± 25.8 s

126.7 ± 27.2*s

65.9 ± 40.4* s

References:

[1] Bayer RJ, Marbberley DJ, Morton C, Miller CH, Sharma IK, Pfeil BE, Rich S, Hitchcock R, Sykes S. Amer. J Bot. 2009; 96: 668 – 685.

[2] Hunskaar, S., Hole, K. 1987. Pain 30. 103 – 114.