Planta Med 2014; 80 - P2B21
DOI: 10.1055/s-0034-1394898

In vitro anthelmintic activity of Labramia bojeri against Strongyloides venezuelensis

V Rehder 1, L Mourão 1, M Nunes 1, D Stien 2, L Bastos 3, S Allegretti 3
  • 1Research Center for Chemical, Biological and Agricultural CPQBA-UNICAMP, Av Cazellato Alexandre, 999, Paulínia/SP, 13148 – 218, Brazil
  • 2Centre National de la Recherche Scientifique – CNRS – Obsevatoire Océanologique de Banyuls-sur-mer, Avenue du Fontaulé 66650 Banyuls sur mer, France
  • 3Biology Animal Department – Biology Institute – UNICAMP, City University Zeferino Vaz – Campinas/SP – 13083 – 862 Brazil

Strongyloidiasis is a negleted parasitic disease caused by the Strongyloides genus. S. stercoralis has high prevalence in humans, with an estimated 100 million people infected worldwide [1]. The aim of this study was evaluated the in vitro anthelmintic activity extract and fractions of different polarities of Labramia bojeri leaves. The ethanolic extract (EELb) was obtained by extraction in Ultraturrax system using ethanol. The EELb was fractionated by liquid-liquid partition using hexane and water to furnish the hexanic (FHxLb) and aqueous (FALb) fractions. The EELb, FHxLb and FALb were dried and solubilized in PBS 2%. In the strongyloidiasis experimental was used adult females of S. venezuelensis obtained from Rattus norvegicus (Wistar). In culture media (RPMI/antibiotic) containing two parasites, the samples were added at concentrations 0, 50, 100, 200, 300 and 400 µg.mL-1 in triplicate. Ivermectin and RPMI were used as positive and negative control, respectively. After incubation (37 ° C with 5% CO2), the mobility reduction and mortality of the parasites was observed in an inverted optical microscope in the ranges of 1 to 6, 24, 48 and 72 hours. The EELb extract showed a 60% overall mortality rate after 72 hours from 300 µg.mL-1, while the FHxLb 50% mortality within 24 hours from the oral dose of 100 µg.mL-1 and 100% mortality after 72 hours at the same concentration. The FALb showed 84% mortality after 48 hours only in the concentration of 400 mg.mL-1. There was no death in positive or negative control, but in ivermectin group, there was damage in worm motility. The results revealed that the process of liquid-liquid partition was efficient and anthelmintic activity in vitro of the lower polarity fraction (FHxLb) was potentiated. Analyses of FHxLb by GC-MS and TLC showed the presence of compounds of the triterpenes class.

Acknowledgements: São Paulo Research Foundation (FAPESP) – Process n° 2013/05355 – 1 and Cnpq – Process 40/2012 Negleted diseases.

Keywords: Strongyloidiasis, Labramia bojeri, triterpenes, activity

References:

[1] Roxby, A.C., Gottlieb, G.S., Limaye, A.P. Strongyloidiasis in transplant patients. Clin. Infect. Dis. 2009; 1, 1411 – 1423.