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DOI: 10.1055/s-0034-1394893
Wound healing effect of new formulations of EB04NAT and EB04BAN in diabetic animals
Introduction: Amazon region has a diversity of botanical species with high levels of interest in the popular medicine. Copaiba oilresins (COR) are widely used to treat several disorders. Our aim in this study was to investigate the wound healing effect of formulations prepared with COR.
Methods: COR (Copaifera multijuga) was collected in Amazonas/Brazil. Voucher specimen is deposited at UFMA herbarium. Swiss webster mice (males, 30 – 35 g; n = 10 – 12) received alloxan (65 mg/kg, i.v.) and after 7 days, diabetic mice (DM) were used in the cutaneous lesions model [1]. Mice received topical administration of EB04NAT or EB04BAN (100 mg/kg) for 16 consecutive days and photos, to accompany lesion retraction, were obtained on days 0, 3, 7, 10, 14, 16. Images were processed in ImageJ program and lesions area were quantified as arbitrary units (AU). Results are presented as medium ± DP. Statistical analyses were done by ANOVA/Bonferroni (*p < 0.05). Protocols for animal use number are #ICBDFBC-015(COBEA/UFRJ/Brazil).
Results: The results (in AU of lesion) are:
Group/Day |
0 |
3 |
7 |
10 |
14 |
16 |
Naïve |
39.7 ± 31 |
28.6 ± 5.5 |
12.9 ± 3.9 |
6.1 ± 2.3 |
3.5 ± 1.7 |
0 |
DM |
36.5 ± 5.9 |
28.3 ± 6.3 |
19.9 ± 5.3 |
13 ± 4.8 |
4.9 ± 2.2 |
3.8 ± 1.3 |
DM/EB04NAT |
32.7 ± 2.8 |
30.5 ± 7.1 |
13.1 ± 4.8 |
5.3 ± 2.2 |
2.4 ± 1.1* |
0.4 ± 0.3* |
DM/EB04BANA |
32.8 ± 5.1 |
31.7 ± 5.4 |
19.1 ± 4.9 |
5.7 ± 2.4 |
2.1 ± 0.7* |
0.6 ± 0.2* |
Conclusion: EB04NAT and EB04BAN had significant wound healing effects accelerating the shrinkage of the lesion. These effects can be, at least in part, related to the presence of sesquiterpenes hydrocarbons oxygenated sesquiterpenes and acidic sesquiterpenes (i.e., β-caryophylene) as quantified in [2] and can be a new treatment option for poor wound healing in diabetic patients.
Acknowledgements: Alan Minho (technical support), Instituto Vital Brazil (donation of animals), CAPES, CNPq and FAPERJ (financial support).
Keywords: Medicinal plants, wound healing, diabetes, amazon region
References:
[1] Wong et al. 2011. J Biomed Biotechnol; 969618.
[2] Gomes et al. 2007. J Ethnopharmacol; 486 – 492.