Planta Med 2014; 80 - P2B16
DOI: 10.1055/s-0034-1394893

Wound healing effect of new formulations of EB04NAT and EB04BAN in diabetic animals

JL Amorim 1, JB Figueiredo 2, ACF Amaral 3, MA Mpalantinos 3, AS Ramos 3, JLP Ferreira 3, SL Basso 4, JRA Silva 5, CF Benjamim 2, PD Fernandes 1
  • 1Federal University of Rio de Janeiro, Institute of Biomedical Science, Laboratory of Pharmacology of Pain and Inflammation. Av. Carlos Chagas Filho 373, J10. Rio de Janeiro, Brazil
  • 2Federal University of Rio de Janeiro, Institute of Biomedical Science, Laboratory of Immunopharmacology. Av. Carlos Chagas Filho 373, J25. Rio de Janeiro, Brazil
  • 3DQPN, Farmanguinhos, FIOCRUZ. Rio de Janeiro, Brazil
  • 4Laboratory of Chemistry of Natural Products, FUNTAC
  • 5Chemistry Institute, UFAM, Brazil

Introduction: Amazon region has a diversity of botanical species with high levels of interest in the popular medicine. Copaiba oilresins (COR) are widely used to treat several disorders. Our aim in this study was to investigate the wound healing effect of formulations prepared with COR.

Methods: COR (Copaifera multijuga) was collected in Amazonas/Brazil. Voucher specimen is deposited at UFMA herbarium. Swiss webster mice (males, 30 – 35 g; n = 10 – 12) received alloxan (65 mg/kg, i.v.) and after 7 days, diabetic mice (DM) were used in the cutaneous lesions model [1]. Mice received topical administration of EB04NAT or EB04BAN (100 mg/kg) for 16 consecutive days and photos, to accompany lesion retraction, were obtained on days 0, 3, 7, 10, 14, 16. Images were processed in ImageJ program and lesions area were quantified as arbitrary units (AU). Results are presented as medium ± DP. Statistical analyses were done by ANOVA/Bonferroni (*p < 0.05). Protocols for animal use number are #ICBDFBC-015(COBEA/UFRJ/Brazil).

Results: The results (in AU of lesion) are:

Group/Day

0

3

7

10

14

16

Naïve

39.7 ± 31

28.6 ± 5.5

12.9 ± 3.9

6.1 ± 2.3

3.5 ± 1.7

0

DM

36.5 ± 5.9

28.3 ± 6.3

19.9 ± 5.3

13 ± 4.8

4.9 ± 2.2

3.8 ± 1.3

DM/EB04NAT

32.7 ± 2.8

30.5 ± 7.1

13.1 ± 4.8

5.3 ± 2.2

2.4 ± 1.1*
(51% reduction)

0.4 ± 0.3*
(89.5% reduction)

DM/EB04BANA

32.8 ± 5.1

31.7 ± 5.4

19.1 ± 4.9

5.7 ± 2.4

2.1 ± 0.7*
(57.1% reduction)

0.6 ± 0.2*
(84.2% reduction)

Conclusion: EB04NAT and EB04BAN had significant wound healing effects accelerating the shrinkage of the lesion. These effects can be, at least in part, related to the presence of sesquiterpenes hydrocarbons oxygenated sesquiterpenes and acidic sesquiterpenes (i.e., β-caryophylene) as quantified in [2] and can be a new treatment option for poor wound healing in diabetic patients.

Acknowledgements: Alan Minho (technical support), Instituto Vital Brazil (donation of animals), CAPES, CNPq and FAPERJ (financial support).

Keywords: Medicinal plants, wound healing, diabetes, amazon region

References:

[1] Wong et al. 2011. J Biomed Biotechnol; 969618.

[2] Gomes et al. 2007. J Ethnopharmacol; 486 – 492.