Planta Med 2014; 80 - P2B5
DOI: 10.1055/s-0034-1394882

Chemical constituents of Cymbocarpum erythraeum (DC.) Boiss. and evaluation of its anti-Helicobacter pylori activity

A Manayi 1, S Heidari 2, AR Goahri 1, S Saeidnia 1, H Mighani 2, HRM Esfahani 3
  • 1Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • 2Division of Chemistry, Faculty of Science, Golestan University, Golestan, Iran
  • 3Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Cymbocarpum erythraeum (Apiaceae) is well represented in the Flora Iranica as an endemic species of Iran [1]. Due to the importance of Apiaceae plants for their several bioactive compounds and regarding to the point that there is no phytochemical and biological investigation on C. erythraeum, isolation of the main secondary metabolites as well as anti-Helicobacter pylori evaluation of the plant have been considered in this evaluation. The shade dried parts of the plant were extracted with hexane, methanol and methanol-water (1:1) using percolator apparatus, respectively. Methanol extract was subsequently fractioned by petroleum ether, butanol and water solvents. The butanol fraction was purified using different column chromatography (CC) methods yielding compounds 1, 2 and 3. The hexane fraction was also purified using CC to provide compound 4. The compounds were identified by 1H-NMR and 13C-NMR methods as isoquercetin (1), rutin (2), β-sitosterol (3) and 2-decenol (4) [2 – 4]. The anti-H. pylori evaluation of the plant extracts and its flavonoids were performed employing disk diffusion method against three clinical isolates of H. pylori revealed that hexane extract of the plant inhibited all clinical isolates of H. pylori. However, methanol (464 mg/mL) and petroleum ether (226 mg/mL) extracts were effective just against one H. pylori strain with inhibition zone diameter values of 14 ± 0.04 and 15 ± 0.02 mm, respectively. However, other fractions and flavonoids did not exhibited antibacterial activity against pathogen bacteria. Due to the best of our knowledge, this is the first report of phytochemical contents and anti-H. pylori activity of the plant.

Keywords: Cymbocarpum erythraeum, Apiaceae, isoquercetin, rutin, Helicobacter pylori

References:

[1] Rechinger KH. Flora Iranica, Umbelliferae. Austria: Akademische Druck-u; 1984.

[2] Gohari AR, Saeidnia S, Shahverdi AR, Yassa N, Malmir M, Mollazade K, Naghinejad AR. Phytochemistry and antimicrobial compounds of Hymenocrater calycinus. Eur Asia J Bio Sci 2009; 3(9): 64 – 68.

[3] Saeidnia S, Gohari AR, Malmir M, Moradi-Afrapoli F, Ajani Y. Tryptophan and sterols from Salvia limbata. J Med Plants 2011; 10(37): 41 – 44.

[4] Manayi A, Saeidnia S, Ostad SN, Hadjiakhoondi A, Shams Ardekani MR, Vazirian M, Akhtar Y, Khanavi M. Chemical constituents and cytotoxic effect of the main compounds of Lythrum salicaria L. Znaturforsch 2013. 68c: 367 – 375.