Planta Med 2014; 80 - P2P14
DOI: 10.1055/s-0034-1394849

The comparative effect of rice bran extract on rats fed a high-fat diet for 4 versus 16 weeks

N Munkong 1, S Sireeratawong 2, A Wongnoppavich 3, N Lerdvuthisopon 2
  • 1Graduate Studies, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
  • 2Institute of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
  • 3Department of Biochemistry, Faculty of Medicine, Chiangmai University, Chiangmai 50100, Thailand

Rice bran water extract (RBE) was shown to reduce fat mass and insulin resistance in Sprague Dawley rats fed a high-fat diet for 4 wks [1, 2]. The effect was further explored for a longer period of high-fat feeding, 16 wks. There were 16 rats in each of 3 groups and 8 rats in the forth one. Rats in groups 2 to 4 were fed with a high-fat diet (HF). The control group was fed with standard chow. Group 3 and 4 were also co-fed daily with 2,205 mg RBE/kg rat wt or 14.9 mg fenofibrate/kg rat wt, respectively. After 4 wks, three satellites, 8 rats each, from group 1 to 3 was extended to 16 wks. There was no satellite in the forth group since fenofibrate was practically prescribed for a short period of time only. Oral glucose tolerance was tested at the end of study. After 4 weeks, the mean ± SEM of liver, abdominal fat and epididymal fat weight (g/100 g rat weight) of the HF group were significantly (p < 0.05) higher than those of control (3.59 ± 0.06 vs. 3.27 ± 0.08, 2.17 ± 0.08 vs. 1.29 ± 0.07, 2.05 ± 0.05 vs. 1.48 ± 0.04, respectively) but reduced to near control when rats were co-fed with either RBE or fenofibrate. The increased liver weight was coincided with their mg triglyceride per g tissue (32.59 ± 2.69 vs. 12.56 ± 0.92). The level reduced when rats were co-fed with either RBE or fenofibrate. RBE could neither reduce organs weight nor liver triglyceride in the 16 wks study. In the HF group, gene expression of nuclear factor kappa B was increased in rat heart muscle within 4 wks and reduced when rats were co-fed with either RBE or fenofibrate. In conclusion, fatty liver was the early and consistent sign of HF insult. RBW was not effective when HF feeding was extended to 16 wks. A trend of chronic inflammation in heart muscle of rats in the HF group was evidence.

Keywords: Fatty liver, tissue triglyceride, chronic inflammation, heart muscle

References:

[1] Kandee N., Sireeratawong S., Burawat B., Utama-Ang N. and Lerdvuthisopon N. The effect of rice bran extract on insulin resistance in high fat diet-induced obese rats. Thammasat Med J, 2009, 9, 140 – 7.

[2] Charkhonpunya C., Sireeratwong S., Komindr S. and Lerdvuthisopon N. Effect of rice-bran water extract on energy metabolism in rats fed a high-fat diet. Thamm Med J, 2011, 11, 221 – 30.