Planta Med 2014; 80 - P1L159
DOI: 10.1055/s-0034-1394816

Isolation and identification of ent-labdan diterpenes from the Mexican plant Gymnosperma glutinosum (Asteraceae) and cytotoxicity evaluation against murine lymphoma L5178Y-R

R Morado-Castillo 1, 2, R Quintanilla-Licea 2, R Gomez-Flores 2, W Blaschek 1
  • 1Pharmazeutisches Institut, Christian-Albrechts-Universität zu Kiel, Gutenbergstraße 76, 24118 Kiel, Germany
  • 2Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N, San Nicolás de los Garza, Nuevo León, Mexico

Cancer is a major health problem worldwide with 14.1 million new cases and 8.2 million deaths in 2012 [1]. Chemotherapy still is an important tool for its treatment and therefore, new anticancer compounds are necessary to contribute to the drug repertoire. Gymnosperma glutinosum is a shrub distributed in Guatemala, Mexico and southeastern of United States. The research aim was to identify compounds from leaves of G. glutinosum and to evaluate their cytotoxic activity vs. murine lymphoma L5178Y-R. Leaves were collected in Escobedo, Nuevo Leon, Mexico and extracted with hexane. After fractionation using common chromatographic methods (Silica Gel, Sephadex LH-20) two compounds (1 and 2) were isolated and analyzed by IR spectroscopy, 1D- and 2D NMR methods as well as HR-MS spectrometry; chirality was determined by measurement of optical activity (comparing with literature) and structural relationships with G. glutinosum's previously reported natural products. Both compounds were identified as ent-labdan diterpenes, diterpene 2 was not reported before for G. glutinosum. Cytotoxic activity against murine lymphoma L5178Y-R was evaluated in vitro by MTT assay. Diterpene 1 showed 44.5% cytotoxicity at 62.5 µg/mL; for 2 no significant cytotoxicity was observed. These results contribute to the scientific Acknowledgement of traditional medicinal plants used in Mexico.

Acknowledgements: We'd like to thank the CONACYT (Mexico) for grant 229170 to Rolando Morado-Castillo and to Prof. Hartmut Laatsch (University of Göttingen) and Prof. Noemi Waksman (UANL) for spectroscopic measurements.

Keywords: Gymnosperma glutinosum, ent-labdan diterpenes, cytotoxicity, L5178Y-R murine lymphoma

References:

[1] Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray, F. Cancer Incidence and Mortality Worldwide: IARC CancerBase No. 11. Available at http://globocan.iarc.fr. Accessed February 26, 2014.