Planta Med 2014; 80 - P1L50
DOI: 10.1055/s-0034-1394707

Chemotaxonomic and biological activities of Tunisian Eryngium species

A Landoulsi 1, 2, 3, A Dussart 1, V Roumy 1, 2, J Bero 5, J Samaillie 1, 2, R Sahli 1, 2, C Rivière 1, 2, S Sahpaz 1, 2, J Quetin-Leclercq 5, C Neut 4, JB Hamida 3, T Hennebelle 1, 2
  • 1EA GRIIOT, EA 4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France
  • 2Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France
  • 3Unité de protéomie fonctionnelle et biopréservation alimentaire, Institut supérieur des sciences biologiques appliquées de Tunis (ISSBAT), Campus Universitaire El Manar, BP 94, Tunis cedex 1068, Tunisia
  • 4INSERM U 995, Laboratoire de Bactériologie, Université Lille 2, 59000 Lille, France
  • 5Université catholique de Louvain, Louvain Drug Research Institute, Pharmacognosy Research Group, Avenue E. Mounier, B1 72.03, B-1200 Brussels, Belgium

The essential oils of six Tunisian Eryngium species obtained by hydrodistillation (Clevenger apparatus) were analyzed by coupled Gas Chromatography-Mass Spectrometry (GC-MS). Falcarinol (0.6 – 98%), terpenes mainly sesquiterpenes (11 – 66%), and mono- and di-terpenes (0.1 – 2.6%) as minor constituents, have been identified. The petroleum ether, dichloromethane and methanol extracts of aerial parts and roots of these species were tested against 36 bacteria and yeast using the microdilution assay and their cytotoxicity was evaluated. The petroleum ether extract of roots of E. triquetrum was the most active with an IC50 up to 0.07 mg/ml. Bio-guided fractionation of this extract was conducted and led to the isolation of seven compounds. Photoactivation[1] of the plant extracts has also been investigated and irradiation with UVA wavelengths results in a higher, light-enhanced, antimicrobial activity.

Keywords: Eryngium, GC-MS, Antimicrobial activity, Photoactivation

References:

[1] Hudson J. B, Towers G. H. N. Therapeutic potential of plant photosensitizers. Pharmac Ther 1991; 49: 181 – 222