In vitro α-glucosidase inhibitory activity of endophytic Alternaria sp. S8 isolated from Morus alba L.

L Zheng; Y Ma; Y Zhang

doi:10.1055/s-0034-1394607

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Planta Med 2014; 80 - P1N17
DOI: 10.1055/s-0034-1394607

In vitro α-glucosidase inhibitory activity of endophytic Alternaria sp. S8 isolated from Morus alba L.

L Zheng ¹, Y Ma ², Y Zhang ³

¹School of Architecture and Urban Environment, Soochow University, Suzhou, 215123, China
²School of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China
³School of Biology & Basic Medical Sciences, Soochow University, Suzhou, 215123, China

Congress Abstract

A total of 106 strains of endophytic fungi were isolated from mulberry tree. 86 strains of the total isolates belonged to 18 genera. The dominant species were Fusarium spp. (22.6%) and Alternaria spp. (15.1%). Identification for the selected isolates was based on 16S rDNA sequences. The potential antidiabetic activity of ethyl acetate extract from Alternaria sp. S8 was investigated by α-glucosidase inhibition assay. The ethyl acetate extracts showed better activity than that of positive control acarbose with IC₅₀ 56.25 µg/ml. Double-reciprocal plots of enzyme kinetics demonstrated an uncompetitive inhibition of α-glucosidase activity by the extracts. Our study demonstrated endophytic microorganisms from M. alba may produce secondary metabolites as new types of α-glucosidase inhibitors.