Int Arch Otorhinolaryngol 2014; 18 - a2330
DOI: 10.1055/s-0034-1388688

Concentration of Elastic Fibers in the Lamina Propria of the Vocal Folds in the Elderly

Adriana Bueno Benito Pessin 1, Anete Branco 1, Selma Maria Miquelim Matheus 1, Tatiana Maria Gonçalves 1, Regina Helena Garcia Martins 1
  • 1Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp)

Introduction: One of the structural changes in the lamina propria of vocal folds in the elderly is related to the concentration of elastic fibers, a major component of the extracellular matrix.

Objective: To quantify by morphometric analysis the changes in the concentration of elastic fibers-related presbyphonia.

Methods: A total of 30 vocal folds (male—15, female—15) were removed during necropsy and were allocated to three groups: one control group (n = 10) and two groups aged 60-75 (n = 10) and 76-90 years (n = 10). The vocal folds were processed and stained by Calleja. Images were photographed with the axion-vision program and morphometry performed by AVSoft BioView program, calculating the concentration of elastic fiber (in percent) in four non-overlapping fields of superficial and deep layers of the lamina propria of each vocal fold.

Results: It was observed that with advancing age, there is a decrease in the concentration of elastic fibers in both layers (deep layer—CG-23.53%; 60-75 years 20.79% and 76-90 years 21.14%; and superficial layer GC 11.06%; 60-75 years 9.36% and 76-90 years 9.18%). Regarding gender, the decrease of elastic fibers was more prevalent in females (male: deep layer—CG 22.02%; 60-75 years 20.08% and 76-90 years 21.36%; and the superficial layer—GC 10.23%; 60-75 years 9.77% and 76-90 years 9.45%; and female: deep layer—GC 25.05%; 60-75 years 21.5% and 76-90 years 20.93%; and the superficial layer—GC 11.90%; 60-75 years 8.97% and 76-90 years 8.91%).

Conclusion: There was a decrease in the concentration of elastic fibers with increasing age in both genders, mainly in women.