Curcumin Infusion Effect on Aquaporins-1,4 Expression in Choroid Plexus of Rat's Brain

Recent studies confirm the osmolarity of extracellular fluid in the brain faces significant changes under physiological conditions such as hyponatremia, brain edema, and migraine. It leads to an increase in the brain water content and swelling or edema. Aquaporin1 (AQP1) and Aquaporin4 (AQP4) are playing the main role in water transfer in epithelial cells of choroid plexus (CP). Curcumin derived from the rhizome of the herb of Curcuma longa that has been identified as the active principle of turmeric. It is able to pass the blood-brain barrier. The anti-inflammatory, thrombosis surpassing, hypoglycemic effects of curcumin are also well established. Method: Male Wistar rats with age 5 to 7 weeks divided into three groups: Sham group with DMSO treatment (solvent control), control group (without any treatment), and experimental group. Rats in the experimental group treated with 300 mg/kg curcumin via edible administration. The test repeated in 10 days. After treatment period, the expression of AQP1,4 were assessed histological method and immunohistochemistry (IHC) technique. Results: The results illustrate that curcumin does not change morphological properties of lateral ventricles. Moreover, H&E staining proved curcumin has not had any effect on viability of CP's cells. But in the IHC test we observed AQP1,4 expression in samples that were treated with curcumin had a significant decrease in comparison to sham and control samples.