Polysaccharides of Dendrobium officinale Induce Aquaporin 5 Translocation by Activating M3 Muscarinic Receptors

Xiang Lin; Jingyi Liu; Wingyan Chung; Stephen Cho-Wing Sze; Haiyan Li; Lixing Lao; Yanbo Zhang

doi:10.1055/s-0034-1383411

Planta Medica, Table of Contents

Planta Med 2015; 81(02): 130-137
DOI: 10.1055/s-0034-1383411

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Polysaccharides of Dendrobium officinale Induce Aquaporin 5 Translocation by Activating M3 Muscarinic Receptors

Xiang Lin

¹School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

,

Jingyi Liu

¹School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

,

Wingyan Chung

¹School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

,

Stephen Cho-Wing Sze

¹School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

,

Haiyan Li

²Foshan University, Department of Pharmacy, Foshan, China

³Guangdong Guofang Medical Technology Co. Ltd., Dongguan, China

,

Lixing Lao

¹School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

,

Yanbo Zhang

¹School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

› Author Affiliations

Abstract

Dendrobium officinale is an herbal medicine that has been clinically used to promote body fluid production. Previous works demonstrated that D. officinale polysaccharides could ameliorate symptoms of salivary secretion of patients with Sjögrenʼs syndrome and in a respective mice model. In the present study, we investigated the underlying mechanism by which D. officinale polysaccharides activate M3 muscarinic receptors and induce extracellular calcium influx, leading to the translocation of aquaporin 5, a water channel protein, to the apical membrane of human submandibular gland epithelial cells. Enzymatic treatment of D. officinale polysaccharides suggested that they are hydrolyzed but do not permeate cell membranes. This finding supports the pharmacological activity of D. officinale polysaccharides to promote salivary secretion.

Key words

Dendrobium officinale - Orchidaceae - polysaccharides - M3 muscarinic receptor - aquaporin 5

Full Text

References

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