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Planta Med 2014; 80(18): 1692-1697
DOI: 10.1055/s-0034-1383146
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antiproliferative Activity of Artemisia asiatica Extract and Its Constituents on Human Tumor Cell Lines

Zsuzsanna Hajdú
1   Institute of Pharmacognosy, University of Szeged, Szeged, Hungary
,
Judit Hohmann
1   Institute of Pharmacognosy, University of Szeged, Szeged, Hungary
,
Peter Forgo
1   Institute of Pharmacognosy, University of Szeged, Szeged, Hungary
,
Imre Máthé
1   Institute of Pharmacognosy, University of Szeged, Szeged, Hungary
2   Institute of Ecology and Botany, Centre for Ecological Research, Hungarian Academy of Sciences, Vácrátót, Hungary
,
Judit Molnár
3   Institute of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary
,
István Zupkó
3   Institute of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary
› Author Affiliations
Further Information

Publication History

received 19 May 2014
revised 09 September 2014

accepted 15 September 2014

Publication Date:
08 October 2014 (online)

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Abstract

The extract of Artemisia asiatica herb with antiproliferative activity against four human tumor cell lines (A2780, A431, HeLa, and MCF7) was analyzed by the MTT assay, and bioassay-directed fractionation was carried out in order to identify the compounds responsible for the cytotoxic activity. Guaianolide (1–4), seco-guianolide (5), germacranolide (6) and eudesmanolide sesquiterpenes (7), monoterpenes (8, 9), including the new compound artemisia alcohol glucoside (8), and flavonoids (10–16) were isolated as a result of a multistep chromatographic procedure (CC, CPC, PLC, and gel filtration). The compounds were identified by means of UV, MS, and NMR spectroscopy, including 1H-and 13C-NMR, 1H-1H COSY, NOESY, HSQC, and HMBC experiments. The isolated compounds 1–16 were evaluated for their tumor cell growth-inhibitory activities on a panel of four adherent cancer cell lines, and different types of secondary metabolites were found to be responsible for the cytotoxic effects of the extract. Especially cirsilineol (13), 3β-chloro-4α,10α-dihydroxy-1α,2α-epoxy-5α,7αH-guai-11(13)-en-12,6α-olide (3), and iso-seco-tanapartholide 3-O-methyl ester (5) exerted marked cytotoxic effects against the investigated cell lines, while jaceosidin (12), 6-methoxytricin (15), artecanin (2), and 5,7,4′,5′-tetrahydroxy-6,3′-dimethoxyflavone (14) were moderately active. All the sesquiterpenes and monoterpenes are reported here for the first time from this species, and in the case of artecanin (2), 3α-chloro-4β,10α-dihydroxy-1β,2β-epoxy-5α,7αH-guai-11(13)-en-12,6α-olide (4), ridentin (6), and ridentin B (7), previously unreported NMR spectroscopic data were determined.

Key words

Artemisia asiatica - Asteraceae - antiproliferative activity - sesquiterpene lactones - monoterpenes - flavonoids

Supporting Information

 

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