Vestitol and neovestitol from Brazilian red propolis reduce leukocytes adhesion in the inflammatory process

Apis mellifera propolis is a natural product that has been subject of extensive studies in recent decades. This study aimed to evaluate the activity of the isolated compounds vestitol and neovestitol (isoflavones) from Brazilian red propolis on modulation of neutrophil migration and adhesion in the inflammatory process. The isolation of vestitol and neovestitol from propolis was performed by HPLC-RP. C57BL/6/JUnib mice were divided into groups: vestitol and neovestitol (1, 3 or 10 mg/kg, sc) for neutrophils recruitment assays induced by LPS or mBSA and observed by intravital microscopy. ANOVA followed by Tukey test (p < 0.05) were applied for statistical analyses. Vestitol and neovestitol decreased neutrophil migration into the peritoneal cavity induced by mBSA or LPS (p < 0.05). It was also found that both compounds reduced the adhesion of leukocytes to venular endothelium of mesenteric microcirculation in mice (p < 0.05). Thus, we concluded that vestitol and neovestitol decreased migration and adhesion of leukocytes in the inflammatory process, suggesting that these compounds could be promising anti-inflammatory agents. Supported by FAPESP/SP/Brazil: 2012/01365 – 0 and 2012/22378 – 2.