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DOI: 10.1055/s-0034-1382531
Structural characterization of dendrobine-type sesquiterpene alkaloids from the stems of Dendrobium nobile using LC-MS/MS
Dendrobium nobile has gained attention in pre-workout products since alkaloids from this plant were claimed to enhance physical or athletic performance. Dendrobine-type sesquiterpene alkaloids were identified via diagnostic fragments using UHPLC/ESI-QTOFMS. Structures of alkaloids were characterized on the basis of accurate mass spectra from QTOF mass analyzer in MS and MS-MS tandem modes. Nine alkaloids were separated within 12 minutes using a C18 column. The fragmentation pathways of two sub-group compounds (dendrobine and nobilonine) were illustrated. This developed method can identify the alkaloid markers lacking of ultraviolet absorption, and is suitable as a quality control method for various Dendrobium samples and commercial products. Alkaloids were identified from D. nobile, but not detected from 12 other Dendrobium species and the 13 dietary supplements.