Isolation of Astragaloside IV and Cyclocephaloside I from Astragalus gummifera and evaluation of Astragaloside IV ON CCL4 induced liver damage in rats

The aim of the current studies was to isolate to Astragaloside IV and Cyclocephaloside I from the roots of A. gummifera and Astragaloside IV was evaluted for hepatoprotective activities. The column chromatography was used for isolation of Astragaloside IV and Cyclocephaloside I and identified by NMR. Astragaloside IV was used to determine the CCl₄ induced toxicity for safe use in hepatic ailment. The dose of 25, 50 and 75 mg/kg of Astragaloside IV subjected for hepatoprotective effects, where 36 Wistar albino rats were equally divided into 6 groups. Injection of a single dose of CCl₄ produced a significant changed in hepatotoxicities and nephrotoxicities biomarkers. The result of present studies revealed the structure of the Astragaloside IV and Cyclocephaloside I a type of saponins were identified with the help of ¹³C-NMR, ¹H-NMR, HMBC, HMQC, NOESY and COSY. With the treatment of 25, 50 and 75 mg/kg of both Astragaloside IV reverse the liver diagnostic stricture of nephrotoxicities and hepatotoxicities. Comparatively the dose 75 mg/kg was highly significant (***P < 0.001), whereas 25 mg/kg was a less significant (*P < 0.05) hepatoprotective agent. In conclusion, saponine like Astragaloside IV showed a significant hepatoprotective compounds. Further preservative studies are required on more rats and on higher animals for more support of present finding.