Anti-biofilm and kinetic studies of kaurane diterpenes that targets oral anaerobes

CHG Martins; T da Silva Moraes; F Abrão; SR Ambrósio; RC Sola Veneziani

doi:10.1055/s-0034-1382470

Planta Medica, Table of Contents

Anti-biofilm and kinetic studies of kaurane diterpenes that targets oral anaerobes

CHG Martins ¹, T da Silva Moraes ¹, F Abrão ¹, SR Ambrósio ¹, RC Sola Veneziani ¹

¹Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Av. Dr. Armando Salles de Oliveira 201, 14404 – 600 Franca-SP, Brazil

Abstract

In previous studies performed by our research group, it was evaluated the antibacterial activity of 12 ent-kaurane diterpenes against 14 bacteria isolated from endodontic infections. ent-Kaurenoic acid (1) and its sodium salt (2) displayed very promising MIC values (lower than 10 µg/mL) for Porphyromas gingivalis and Peptostreptococcus micros. Due to these results, antibiofilm and time-kill curves studies with 1 and 2 were performed against P. gingivalis and P. micros. Minimal Biofilm Inhibitory Concentrations (MBIC₅₀) of 12.5 and 50 µg/mL were observed respectively for 1 and 2 against P. gingivalis. A 12.5 µg/mL MBIC₅₀value was achieved for both diterpenes when tested on P. micros. Kinetic studies (time-kill curve) allowed us to observe that 1 and 2 were able to completely kill P. gingivalis within 72 and 48 hours respectively at the same concentrations (3.12 µg/mL). P. micros showed to be more sensitive in the presence of both diterpenes, being eliminated within 12 hours (1; 3.12 µg/mL) and 18 hours (2; 6.25 µg/mL).

Fig. 1

The authors tank to FAPESP for funds and grants (process number 2012/25237 – 0)