Planta Med 2014; 80 - PD32
DOI: 10.1055/s-0034-1382453

New anthraquinones with in vitro binding affinity for cannabinoid receptors from Asphodelus microcarpus

MM Ghoneim 1, 4, AA El-Hela 4, AEI Mohammad 4, E El-Ghaly 4, SJ Cutler 3, SA Ross 1, 2
  • 1National Center for Natural Products Research
  • 2Department of Pharmacognosy
  • 3Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
  • 4Department of Pharmacognosy, Faculty of Pharmacy, University of Al-Azhar, Cairo 11371, Egypt

Bioassay guided fractionation of the ethanolic extract of Asphodelus microcarpus Salzm.et Viv. (Xanthorrhoeaceae or Asphodelaceae) resulted in the isolation of four new compounds identified as asphodosides A-D. The isolated metabolites showed high binding affinity to cannabinoid receptors (subtype CB1 and CB2).

Tab. 1: The binding affinity of isolated compounds

Sample Name

MW

uL of DMSO

Concentration Tested

CB1%Inhibition

CB2%Inhibition

Asphodocide A

640

78

10 uM

78.9

14.8

Asphodocide B

640

156

10 uM

78.3

41.2

Asphodocide C

640

78

10 uM

110.0

17.5

Asphodocide D

640

234

10 uM

62.7

27.1