Planta Med 2014; 80 - PD3
DOI: 10.1055/s-0034-1382424

Phytochemical investigation and pharmacological evaluation of Pulsatilla patens var. Patens

VK Sharma 1, G Łaska 2, SVS Radhakrishnan 3, MR Jacob 3, JK Zjawiony 1
  • 1Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA
  • 2Bialystok University of Technology, Wiejska 45A, 15 – 351, Bialystok, Poland
  • 3National Center for Natural Product Research, The University of Mississippi, University, MS 38677, USA

Pulsatilla patens (L.) Mill. belongs to Buttercup family (Ranunculaceae) commonly known as American Pasqueflower and Anemone patens L. This flowering plant is native to Europe, Russia, Mongolia, China, Canada and United States. Three distinguished subspecies of P. patens are P. patens subsp. patens (L.) Mill., subsp. multifida (G. A. Pritzel) Zamels and subsp. flavescens (Zucc.) Zamels. Due to the synergy between the prairie crocus and shortgrass prairie ecosystems the population of P. patens is declining. Ethanolic extract of the roots of P. patens subsp. patens showed significant antifungal activity. The crude extract (0.9 grams) of the plant was subjected to column chromatography which resulted in the isolation of two known oleanane-type triterpene glycosides. Detailed procedure of isolation, spectroscopic identification and pharmacological evaluation of compounds will be presented.