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Planta Med 2014; 80(08/09): 655-661
DOI: 10.1055/s-0034-1368544
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Inhibitory Effects of β-Chamigrenal, Isolated from the Fruits of Schisandra chinensis, on Lipopolysaccharide-Induced Nitric Oxide and Prostaglandin E2 Production in RAW 264.7 Macrophages

Ji-Sun Shin
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
5   Reactive Oxygen Species Medical Research Center, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
6   Department of Physiology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
,
Suran Ryu
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
2   Department of Biomedical Science, College of Medical Science, Kyung Hee University, Seoul, Republic of Korea
,
Young-Wuk Cho
2   Department of Biomedical Science, College of Medical Science, Kyung Hee University, Seoul, Republic of Korea
5   Reactive Oxygen Species Medical Research Center, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
6   Department of Physiology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
,
Hyun Ji Kim
3   Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
,
Dae Sik Jang
4   Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
,
Kyung-Tae Lee
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
4   Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 02 December 2013
revised 09 April 2014

accepted 05 May 2014

Publication Date:
28 May 2014 (online)

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Inhibitory Effects of β-Chamigrenal, Isolated from the Fruits of Schisandra chinensis, on Lipopolysaccharide-Induced Nitric Oxide and Prostaglandin E2 Production in RAW 264.7 MacrophagesPlanta Med 2014; 80(08/09): E1-E1
DOI: 10.1055/s-0034-1368636

Abstract

Much is known about the bioactive properties of lignans from the fruits of Schisandra chinensis. However, very little work has been done to determine the properties of sesquiterpenes in the fruits of S. chinensis. The aim of the present study was to investigate the anti-inflammatory potential of new sesquiterpenes (β-chamigrenal, β-chamigrenic acid, α-ylangenol, and α-ylangenyl acetate) isolated from the fruits of S. chinensis and to explore their effect on macrophages stimulated with lipopolysaccharide. Of these four sesquiterpenes, β-chamigrenal most significantly suppressed lipopolysaccharide-induced nitric oxide and prostaglandin E2 production in RAW 264.7 macrophages (47.21 ± 4.54 % and 51.61 ± 3.95 % at 50 µM, respectively). Molecularly, the inhibitory activity of β-chamigrenal on nitric oxide production was mediated by suppressing inducible nitric oxide synthase activity but not its expression. In the prostaglandin E2 synthesis pathway, β-chamigrenal prevented the upregulation of inducible microsomal prostaglandin E synthase-1 expression after stimulation with lipopolysaccharide. Conversely, β-chamigrenal had no effect on the expression and enzyme activity of cyclooxygenase-2. In addition, the expression of early growth response factor-1, a key transcription factor of microsomal prostaglandin E synthase-1 expression, was inhibited by β-chamigrenal. These results may suggest a possible anti-inflammatory activity of β-chamigrenal which has to be proven in in vivo experiments.

Key words

Schisandrae Fructus - Schisandra chinensis - Schisandraceae - sesquiterpene - nitric oxide - prostaglandin E2 - mPGES-1

Supporting Information

 

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