Download PDF
Planta Med 2014; 80(07): 544-549
DOI: 10.1055/s-0034-1368445
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Aurantio-Obtusin Stimulates Chemotactic Migration and Differentiation of MC3T3-E1 Osteoblast Cells

Chethala N. Vishnuprasad
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
,
Tomoko Tsuchiya
2   YU-ECI Research Center for Medical Sciences, Yeungnam University, Gyeongsan, Republic of Korea
3   Dept. of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu, Republic of Korea
,
Shiro Kanegasaki
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
2   YU-ECI Research Center for Medical Sciences, Yeungnam University, Gyeongsan, Republic of Korea
,
Joon Ho Kim
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
,
Sung Soo Han
1   Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, Gyeongsan, Republic of Korea
2   YU-ECI Research Center for Medical Sciences, Yeungnam University, Gyeongsan, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 03 December 2013
revised 31 March 2014

accepted 01 April 2014

Publication Date:
19 May 2014 (online)

    Permissions and Reprints

Abstract

Osteoporosis is one of the major metabolic bone diseases and is among the most challenging noncommunicable diseases to treat. Although there is an increasing interest in identifying bioactive molecules for the prevention and management of osteoporosis, such studies principally focus only on differentiation and mineralization of osteoblasts or inhibition of osteoclast activity. Stimulation of osteoblast migration must be a promising osteoanabolic strategy for improved metabolic bone disease therapy. In this study, we show that an anthraquinone derivative, aurantio-obtusin, stimulated chemotactic migration of MC3T3-E1 osteoblast cells in a concentration-dependent manner. The use of a real-time chemotaxis analyzing system, TAXIScan, facilitated the evaluation of both velocity and directionality of osteoblast migration in response to the compound. Besides migration, the compound stimulated osteoblast differentiation and mineralization. Taken together, the data presented in this paper demonstrate that aurantio-obtusin is a promising osteoanabolic compound of natural origin with potential therapeutic applications in the prevention of osteoporosis and other metabolic bone diseases.

Key words

aurantio-obtusin - TAXIScan - chemotaxis - osteoblast migration - differentiation - mineralization
 

  • References

  • 1 Proff P, Romer P. The molecular mechanism behind bone remodelling: a review. Clin Oral Investig 2009; 13: 355-362
    CrossrefPubMedGoogle Scholar
  • 2 Handa R, Ali Kalla A, Maalouf G. Osteoporosis in developing countries. Best Pract Res Clin Rheumatol 2008; 22: 693-708
    CrossrefPubMedGoogle Scholar
  • 3 Holroyd C, Cooper C, Dennison E. Epidemiology of osteoporosis. Best Pract Res Clin Endocrinol Metab 2008; 22: 671-685
    CrossrefPubMedGoogle Scholar
  • 4 Lane NE. Epidemiology, etiology, and diagnosis of osteoporosis. Am J Obstet Gynecol 2006; 194: S3-S11
    CrossrefPubMedGoogle Scholar
  • 5 International Osteoporosis Foundation. Facts and statistics. Available at. http://www.iofbonehealth.org/facts-statistics Accessed on March 26, 2014
    PubMedGoogle Scholar
  • 6 Shan PF, Xian CJ, Li M, Xiang GD, Yuan LQ. Osteoporosis. Int J Endocrinol 2013; 2013: 2
    PubMedGoogle Scholar
  • 7 Warriner AH, Saag KG. Osteoporosis diagnosis and medical treatment. Orthop Clin North Am 2013; 44: 125-135
    CrossrefPubMedGoogle Scholar
  • 8 Mitchell BD, Streeten EA. Clinical impact of recent genetic discoveries in osteoporosis. Appl Clin Genet 2013; 6: 75-85
    PubMedGoogle Scholar
  • 9 Richards JB, Zheng HF, Spector TD. Genetics of osteoporosis from genome-wide association studies: advances and challenges. Nat Rev Genet 2012; 13: 576-588
    CrossrefPubMedGoogle Scholar
  • 10 Das S, Crockett JC. Osteoporosis – a current view of pharmacological prevention and treatment. Drug Des Devel Ther 2013; 7: 435-448
    PubMedGoogle Scholar
  • 11 Rachner TD, Khosla S, Hofbauer LC. Osteoporosis: now and the future. Lancet 2011; 377: 1276-1287
    CrossrefPubMedGoogle Scholar
  • 12 Baron R, Hesse E. Update on bone anabolics in osteoporosis treatment: rationale, current status, and perspectives. J Clin Endocrinol Metab 2012; 97: 311-325
    CrossrefPubMedGoogle Scholar
  • 13 Beamer B, Hettrich C, Lane J. Vascular endothelial growth factor: an essential component of angiogenesis and fracture healing. HSS J 2010; 6: 85-94
    CrossrefPubMedGoogle Scholar
  • 14 Hadjidakis DJ, Androulakis II. Bone remodeling. Ann N Y Acad Sci 2006; 1092: 385-396
    CrossrefPubMedGoogle Scholar
  • 15 Putnam SE, Scutt AM, Bicknell K, Priestley CM, Williamson EM. Natural products as alternative treatments for metabolic bone disorders and for maintenance of bone health. Phytother Res 2007; 21: 99-112
    CrossrefPubMedGoogle Scholar
  • 16 Rayalam S, Yang JY, Della-Fera MA, Baile CA. Novel molecular targets for prevention of obesity and osteoporosis. J Nutr Biochem 2011; 22: 1099-1104
    CrossrefPubMedGoogle Scholar
  • 17 Srivastava K, Tyagi AM, Khan K, Dixit M, Lahiri S, Kumar A, Changkija B, Khan MP, Nagar GK, Yadav DK, Maurya R, Singh SP, Jain GK, Wahajuddin. Trivedi R, Chattopadhyay N, Singh D. Isoformononetin, a methoxydaidzein present in medicinal plants, reverses bone loss in osteopenic rats and exerts bone anabolic action by preventing osteoblast apoptosis. Phytomedicine 2013; 20: 470-480
    CrossrefPubMedGoogle Scholar
  • 18 Ha H, Shim KS, An H, Kim T, Ma JY. Water extract of Spatholobus suberectus inhibits osteoclast differentiation and bone resorption. BMC Complement Altern Med 2013; 13: 112-120
    CrossrefPubMedGoogle Scholar
  • 19 Tiyasatkulkovit W, Charoenphandhu N, Wongdee K, Thongbunchoo J, Krishnamra N, Malaivijitnond S. Upregulation of osteoblastic differentiation marker mRNA expression in osteoblast-like UMR106 cells by puerarin and phytoestrogens from Pueraria mirifica . Phytomedicine 2012; 19: 1147-1155
    CrossrefPubMedGoogle Scholar
  • 20 Dirckx N, Van Hul M, Maes C. Osteoblast recruitment to sites of bone formation in skeletal development, homeostasis, and regeneration. Birth Defects Res C Embryo Today 2013; 99: 170-191
    PubMedGoogle Scholar
  • 21 Kanegasaki S, Nomura Y, Nitta N, Akiyama S, Tamatani T, Goshoh Y, Yoshida T, Sato T, Kikuchi Y. A novel optical assay system for the quantitative measurement of chemotaxis. J Immunol Methods 2003; 282: 1-11
    CrossrefPubMedGoogle Scholar
  • 22 Nitta N, Tsuchiya T, Yamauchi A, Tamatani T, Kanegasaki S. Quantitative analysis of eosinophil chemotaxis tracked using a novel optical device – TAXIScan. J Immunol Methods 2007; 320: 155-163
    CrossrefPubMedGoogle Scholar
  • 23 Yamauchi A, Degawa-Yamauchi M, Kuribayashi F, Kanegasaki S, Tsuchiya T. Systematic single cell analysis of migration and morphological changes of human neutrophils over stimulus concentration gradients. J Immunol Methods 2014; 404: 59-70
    CrossrefPubMedGoogle Scholar
  • 24 Xu L, Chan CO, Lau CC, Yu Z, Mok DK, Chen S. Simultaneous determination of eight anthraquinones in Semen Cassiae by HPLC-DAD. Phytochem Anal 2012; 23: 110-116
    CrossrefPubMedGoogle Scholar
  • 25 Zhang C, Wang R, Liu B, Tu G. Structure elucidation of a sodium salified anthraquinone from the seeds of Cassia obtusifolia by NMR technique assisted with acid-alkali titration. Magn Reson Chem 2011; 49: 529-532
    CrossrefPubMedGoogle Scholar
  • 26 Diao YL, Wang RF, Zhang C, Chen JX, Liu B. A new comprehensive procedure for the quality control of Semen Cassiae and its application in evaluating commercially available material in China. Chin J Nat Med 2013; 11: 433-441
    PubMedGoogle Scholar
  • 27 Ju MS, Kim HG, Choi JG, Ryu JH, Hur J, Kim YJ, Oh MS. Cassiae semen, a seed of Cassia obtusifolia, has neuroprotective effects in Parkinsonʼs disease models. Food Chem Toxicol 2010; 48: 2037-2044
    CrossrefPubMedGoogle Scholar
  • 28 Kim DH, Kim S, Jung WY, Park SJ, Park DH, Kim JM, Cheong JH, Ryu JH. The neuroprotective effects of the seeds of Cassia obtusifolia on transient cerebral global ischemia in mice. Food Chem Toxicol 2009; 47: 1473-1479
    CrossrefPubMedGoogle Scholar
  • 29 Kitanaka S, Nakayama T, Shibano T, Ohkoshi E, Takido M. Antiallergic agent from natural sources. Structures and inhibitory effect of histamine release of naphthopyrone glycosides from seeds of Cassia obtusifolia L. Chem Pharm Bull (Tokyo) 1998; 46: 1650-1652
    CrossrefPubMedGoogle Scholar
  • 30 Grey A, Reid IR. Emerging and potential therapies for osteoporosis. Expert Opin Investig Drugs 2005; 14: 265-278
    CrossrefPubMedGoogle Scholar
  • 31 Lewiecki EM. Combination therapy: the Holy Grail for the treatment of postmenopausal osteoporosis?. Curr Med Res Opin 2011; 27: 1493-1497
    CrossrefPubMedGoogle Scholar
  • 32 Lamers E, van Horssen R, te Riet J, van Delft FC, Luttge R, Walboomers XF, Jansen JA. The influence of nanoscale topographical cues on initial osteoblast morphology and migration. Eur Cell Mater 2010; 20: 329-343
    PubMedGoogle Scholar
  • 33 Pagani F, Francucci CM, Moro L. Markers of bone turnover: biochemical and clinical perspectives. J Endocrinol Invest 2005; 28: 8-13
    CrossrefPubMedGoogle Scholar
  • 34 Hoemann CD, El-Gabalawy H, McKee MD. In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization. Pathol Biol (Paris) 2009; 57: 318-323
    CrossrefPubMedGoogle Scholar
  • 35 Hengartner NE, Fiedler J, Ignatius A, Brenner RE. IL-1beta inhibits human osteoblast migration. Mol Med 2013; 19: 36-42
    PubMedGoogle Scholar
  • 36 Nakasaki M, Yoshioka K, Miyamoto Y, Sasaki T, Yoshikawa H, Itoh K. IGF-I secreted by osteoblasts acts as a potent chemotactic factor for osteoblasts. Bone 2008; 43: 869-879
    CrossrefPubMedGoogle Scholar
  • 37 Dimitriou R, Tsiridis E, Giannoudis PV. Current concepts of molecular aspects of bone healing. Injury 2005; 36: 1392-1404
    CrossrefPubMedGoogle Scholar
  • 38 Giannoudis PV, Einhorn TA, Marsh D. Fracture healing: the diamond concept. Injury 2007; 38: 3-6
    PubMedGoogle Scholar
  • 39 Nam TW, Yoo CI, Kim HT, Kwon CH, Park JY, Kim YK. The flavonoid quercetin induces apoptosis and inhibits migration through a MAPK-dependent mechanism in osteoblasts. J Bone Miner Metab 2008; 26: 551-560
    CrossrefPubMedGoogle Scholar
  • 40 Phan TT, Hughes MA, Cherry GW. Effects of an aqueous extract from the leaves of Chromolaena odorata (Eupolin) on the proliferation of human keratinocytes and on their migration in an in vitro model of reepithelialization. Wound Repair Regen 2001; 9: 305-313
    CrossrefPubMedGoogle Scholar
  • 41 Zhang Q, Fong CC, Yu WK, Chen Y, Wei F, Koon CM, Lau KM, Leung PC, Lau CB, Fung KP, Yang M. Herbal formula Astragali radix and Rehmanniae radix exerted wound healing effect on human skin fibroblast cell line Hs27 via the activation of transformation growth factor (TGF-β) pathway and promoting extracellular matrix (ECM) deposition. Phytomedicine 2012; 20: 9-16
    CrossrefPubMedGoogle Scholar
  • 42 Kim H, Kim J, Park J, Kim SH, Uchida Y, Holleran WM, Cho Y. Water extract of gromwell (Lithospermum erythrorhizon) enhances migration of human keratinocytes and dermal fibroblasts with increased lipid synthesis in an in vitro wound scratch model. Skin Pharmacol Physiol 2012; 25: 57-64
    CrossrefPubMedGoogle Scholar