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Exp Clin Endocrinol Diabetes 2014; 122(03): 179-189
DOI: 10.1055/s-0034-1367004
Article
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Immunomodulatory Properties of Pancreatic Islet-Derived Stem Cells Co-Cultured with T cells: Does It Contribute to the Pathogenesis of Type 1 Diabetes?

A. E. Sariboyaci
1   Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli, Turkey
,
P. C. Demircan
1   Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli, Turkey
,
G. Gacar
1   Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli, Turkey
,
Z. S. Unal
1   Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli, Turkey
,
G. Erman
1   Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli, Turkey
,
E. Karaoz
1   Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli University, Kocaeli, Turkey
› Author Affiliations
Further Information

Publication History

received 08 October 2013
first decision 14 December 2013

accepted 14 January 2014

Publication Date:
11 July 2014 (online)

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Abstract

Background:

Previously, we isolated stem cells from rat pancreatic islets (rPI-SCs) with similar characteristics of bone-marrow derived-mesenchymal stem cells (MSCs). We aimed to investigate the immunomodulatory effects of them on stimulated T-cells.

Methods:

Following in vitro co-culturing directly and indirectly, the response of T-cells stimulated by concanavalin-A and immunosuppressive activity of rPI-SCs were evaluated by analysing in terms of cell viability, proliferation and apoptosis, cell cycle, differentiation of Treg, cytokines and some regulatory factors produced from T and SCs.

Results:

Our results have firstly demonstrated that rPI-SCs like MSCs could regulate stimulated T-cell responses by altering their cell-cycle and cytokine profile, inhibiting the cell proliferation, and inducing the apoptosis and differentiation of Treg. Direct and indirect in vitro co-cultures of rPI-SCs with stimulated T-cells showed immunosuppressive effects.

Conclusion:

Therefore, we are introducing a novel type of stem cell with immunomodulatory properties. On the other hand, it is questionable why PI-SCs cannot protect the insulin producing cells from attacks of autoreactive T-cells in the developing of type1 diabetes. For this purpose, further molecular researches in vitro and in vivo are needed to clarify why PI-SCs may not suppress attacks of autoreactive-immune-cells towards PIs. PI-SCs from diseased people should be compared with pancreas of healthy ones at both genomic and proteomic levels.

Key words

characterization - immunosuppression - pancreatic islets - stem cells
 

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