Circulating CD3⁺56⁺ Cell Subset in Pre-diabetes

Abstract

Background:

The CD3⁺56⁺ cells are a small but significant population of T lymphocytes encompassing NKT-like and NKT cells, which may play the essential role at the very early stages of atherosclerotic plaque development. The frequency and activity of CD3⁺56⁺ cells in atherosclerosis-inducing dysglycaemic disease (diabetes type 2 or pre-diabetes) is largely unknown.

Methods:

We analysed CD3⁺56⁺ cell count, granzyme, perforin and annexin V profiles in the peripheral blood from a group of patients with pre-diabetes, with diabetes type 2 and from non-dysglycaemic controls. Measurements were made of fasting glucose levels, HbA₁c, 1,5-anhydroglucitol and lipid profile.

Results:

The mean counts of CD3⁺56⁺ cells were significantly higher in patients with pre-diabetes compared to both patients with diabetes and to control group. There was an increase in the number of CD3⁺56⁺ cells producing granzyme and perforin in pre-diabetic patients compared to other groups, while there were no difference in annexin V⁺ populations within examined groups. It was confirmed that CD3⁺56⁺ cells count is modified by metabolic factors and their parameters, namely HbA₁c and 1,5-anhydroglucitol values.

Conclusion:

It could be stated that the alterations of CD3⁺ 56⁺  cells count in peripheral blood of pre-diabetic and type 2 diabetic patients are related to different grades of carbohydrate deteriorations – postprandial hyperglycaemia and chronic hyperglycaemia.

Key words

CD3⁺56⁺ cells - cardiovascular risk management - pre-diabetes - diabetes type 2 - 1,5-anhydroglucitol

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