Fast Quantification of S-adenosyl-L-methionine in Dietary
                    Health Products Utilizing Reversed-Phase High-performance Liquid Chromatography:
                    Teaching an Old Method New Tricks

Jing-Ru Liou; Li-Yeh Chuang; Yuan-Han Yang; Tung-Ying Wu; Mohamed El-Shazly; Shyh-Jong Wu; Tusty-Jiuan Hsieh; Yang-Chang Wu; Jiumn-Yih Wu; Fang-Rong Chang

doi:10.1055/s-0033-1360301

Planta Medica, Inhaltsverzeichnis

Planta Med 2014; 80(02/03): 243-248
DOI: 10.1055/s-0033-1360301

Analytical Studies

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Fast Quantification of S-adenosyl-L-methionine in Dietary Health Products Utilizing Reversed-Phase High-performance Liquid Chromatography: Teaching an Old Method New Tricks

Authors

Jing-Ru Liou^*

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
Li-Yeh Chuang^*

²Department of Chemical Engineering, Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan
Yuan-Han Yang

³Department of Neurology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

⁴Department of Masterʼs Program in Neurology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
Tung-Ying Wu

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
Mohamed El-Shazly

⁵Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity, Abassia, Cairo, Egypt
Shyh-Jong Wu

⁶Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan
Tusty-Jiuan Hsieh

⁷Department of Genome Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
Yang-Chang Wu

⁸School of Pharmacy, College of Pharmacy, China Medical University, Taichung, Taiwan

⁹Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, Taiwan

¹⁰Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan
Jiumn-Yih Wu

²Department of Chemical Engineering, Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan
Fang-Rong Chang

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

¹¹Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

¹²Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan

Abstract

S-adenosyl-L-methionine is a ubiquitous methyl donor in living bodies. It is known to participate in several physiological processes including homocysteine metabolism and glutathione synthesis regulation, and cellular antioxidant mechanism. S-adenosyl-L-methionine containing dietary supplements has been prescribed recently for the treatment of depression, arthritis, and liver diseases with encouraging results. The development of an efficient analytical protocol for S-adenosyl-L-methionine containing dietary supplements is crucial for maintaining product quality and consumer health. In this study, the S-adenosyl-L-methionine content of several yeast products and commercial healthy food product samples was quantitatively analyzed utilizing HPLC. The chromatographic separation was achieved on a reversed-phase column and 2 % acetonitrile with a 98 % ammonium-acetate mobile phase under pH 4.5, with a flow rate of 1.0 mL/min. The wavelength used for detection with the UV detector was 254 nm. The total analysis time was short and the target compound showed a well-defined peak. The correlation coefficient of the regression curve showed good linearity and sensitivity with r = 0.999. All experiments were replicated five times and the relative standard deviations as well as the relative error values were all less than 3 %. Moreover, the achieved precision and accuracy values were high with 97.4–100.9 % recovery. Qualitative determination of S-adenosyl-L-methionine in the tested products was achieved using NMR and LC-MS techniques. The developed protocol is robust, fast, and suitable for the quality control analysis of yeast and commercial S-adenosyl-L-methionine products.

Key words

HPLC - S-adenosyl-L-methionine - Saccharomyces cerevisiae (yeast) - LC-MS

Volltext

Referenzen

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