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Planta Med 2014; 80(14): 1161-1170
DOI: 10.1055/s-0033-1360109
Reviews
Georg Thieme Verlag KG Stuttgart · New York

Natural Product Libraries: Assembly, Maintenance, and Screening.

Mark S. Butler
Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Australia
,
Frank Fontaine
Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Australia
,
Matthew A. Cooper
Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Australia
› Author Affiliations
Further Information

Publication History

received 29 August 2013
revised 22 October 2013

accepted 30 October 2013

Publication Date:
05 December 2013 (online)

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Abstract

This review discusses successful strategies and potential pitfalls to assembling a natural product-based library suitable for high-throughput screening. Specific extraction methods for plants, microorganisms, and marine invertebrates are detailed, along with methods for generating a fractionated sub-library. The best methods to store, maintain and prepare the library for screening are addressed, as well as recommendations on how to develop a robust high-throughput assay. Finally, the logistics of moving from an assay hit to pure bioactive compound are discussed.

Key words

natural product - extraction - library - stability - screening
 

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