Drug Res (Stuttg) 2014; 64(5): 225-228
DOI: 10.1055/s-0033-1357203
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Evaluation of Wound Healing Property of Terminalia catappa on Excision Wound Models in Wistar Rats

A. A. Khan
1   Research Scholar, KIET School of Pharmacy, Ghaziabad, India
V. Kumar
2   Department of Pharmacology, KIET School of Pharmacy, Ghaziabad, India
B. K. Singh
2   Department of Pharmacology, KIET School of Pharmacy, Ghaziabad, India
R. Singh
3   Research Scholar, Amity Institute of Pharmacy, Lucknow, India
› Author Affiliations
Further Information

Publication History

received 26 June 2013

accepted 05 September 2013

Publication Date:
16 October 2013 (online)


Wound is defined as the loss of breaking cellular and functional continuity of the living tissues. Management of wounds is frequently encountered with different problems. Drug resistance and toxicity hindered the development of synthetic antimicrobial agents with wound healing activity. Many plants with potent pharmacological activities may offer better treatment options viz. Terminalia chebula, Terminalia bellirica and Phyllanthus emblica formulations have shown healing activities on wounds.

The present study was planned to investigate the wound healing activity of Terminalia catappa on excision wound model in rats. Ointment was prepared by using bark extract of Terminalia catappa in soft paraffin and preservative. Wistar albino rats (200–250 gm) of either sex were used in the present study. A circular wound of 2 cm in diameter was made on the depilated dorsal thoracic region of the rats under ether anesthesia in aseptic conditions. The ointment was applied for 18 days and percent wound closure observed along with the parameters viz. Epithelization, granuloma weight and scar formation. Animals were observed on 3rd, 6th, 9th, 12th, 15th and 18th post-wounding day.

Wound healing activity was compared with that of control and Betadine ointment as standard drug. Animals treated with Terminalia catappa ointment exhibited 97% reduction in wound area as compared to the control animals (81%). Ointment treated wounds were found to induce epithelization faster compared to the control. In conclusion, Terminalia catappa ointment promotes significant wound healing in rats and further evaluation of this activity in humans is suggested.

  • References

  • 1 Patil S, Ghodke DS, Magdum CS et al. Evaluation of healing activity of marketed formulations on excision wounds models in Albino rats. Int J Pharm Tech Res 2009; 1: 500-501
  • 2 Chitra P, Sajithal GB, Chanrakaran G. Influence of Aloe vera on collagen turnover in healing of dermal wounds in rats. Ind J Exp Biol 1998; 36: 896-901
  • 3 Pandey M, Worlikar PS et al. Comparison of wound healing activity of Jethimadh with Triphala in rats. Int J Health & Allied Sciences 2012; 1: 59-63
  • 4 Kumar MS, Kirubanandan S, Sripriya R et al. Triphala promotes healing of infected full-thickness dermal wound. J Surg Res 2008; 144: 94-101
  • 5 Suruse P, Kale MK, Gunde M et al. Evaluation of wound healing activity of Arisaema leschenaltii blume. Der Pharmacia Lettre 2011; 3: 200-206
  • 6 Majumdar M, Nayeem N, Kamath J et al. Evaluation of Tectona grandis leaves for wound healing activity. Pak J Pharm Sci 2007; 20: 120-124
  • 7 Biswas TK, Mukherjee B. Plant medicine of Indian origin for wound healing activity: A review. Ind J Lower Ext Wounds 2003; 2: 123-132
  • 8 Zuhrotun A, Suganda AG, Nawawi A. Phytochemical study of ketapang bark (Terminalia catappa L.). Int. Conf. on Medi. Plants. Surabaya, Indonesia: ICOMP; 2010
  • 9 Chen PS, Li JH. Chemopreventive effect of punicalagin, a novel tannin component isolated from Terminalia catappa, on H-ras-transformed NIH3T3 cells. Toxicol Letters 2006; 163: 44-53
  • 10 Ahmed SA, Swamy BMV, Gopkumar P et al. Anti-diabetic activity of Terminalia catappa Linn. Leaf extracts in alloxan-induced diabetic rats. Iran J Pharmacol & Therap 2005; 4: 36-39
  • 11 Chu SC, Yang SF, Liu SJ et al. In vitro and in vivo antimetastatic effects of Terminalia catappa L. leaves on lung cancer cells. Food and Chemical Toxicology 2007; 45: 1194-1201
  • 12 Liu TY, Ho LK, Tsai YC et al. Modification of mitomycin C-induced clastogenicity by Terminalia catappa L. in vitro and in vivo. Cancer Letters 1996; 105: 113-118
  • 13 Nagappa AN, Thakurdesai PA, Venkat Rao N et al. Antidiabetic activity of Terminalia catappa Linn fruits. J Ethnopharmacol 2003; 88: 45-50
  • 14 Goun E, Cunningham G, Chu D et al. Antibacterial and antifungal activity of Indonesian ethnomedical plants. Fitoterapia 2003; 76: 592-596
  • 15 Pawar SP, Pal SC. Antimicrobial activity of extracts of Terminalia catappa root. Indian J Med Sci 2002; 56: 276-278
  • 16 Fan YM, Xu LZ, Gao J et al. Phytochemical and antiinflammatory studies on Terminalia catappa. Fitoterapia 2004; 75: 253-260
  • 17 Ratnasooriya WD, Dharmasiri MG, Rajapakse RAS et al. Tender leaf extract of Terminalia catappa antinociceptive activity in rats. Pharmaceutical Biology 2002; 40: 60-66
  • 18 Kinoshita S, Inoue Y, Nakama S et al. Antioxidant and hepatoprotective actions of medicinal herb, Terminalia catappa L. from Okinawa Island and its tannin corilagin. Phytomedicine 2007; 14: 755-762
  • 19 Biswas TK, Mukherjee B. Plant medicines of Indian origin for wound healing activity: a review. Int J Low Extrem Wounds 2003; 2: 25-39
  • 20 Adam JS, Richarada FC. Cutaneous wound healing. N Engl J Med 1999; 341: 738-746
  • 21 Abrahamian FM, Goldstein EJC. Microbiology of animal bite wound infections. Clin Microbiol Rev 2011; 24: 231