Evaluation of Dimethyl Sulfoxide and Dexamethasone on Pulmonary Contusion in Experimental Blunt Thoracic Trauma
18. Mai 2013
08. Juli 2013
12. August 2013 (eFirst)
Background A thoracic trauma model was designed to evaluate the effect of dimethyl sulfoxide (DMSO) and dexamethasone (DX) on histopathologic and oxidative changes in lung parenchyma seen after pulmonary contusion.
Methods Twenty-four Wistar albino rats were included in the study. They were allocated into control (CG, n = 6), sham (SG, n = 6), DX (DXG, n = 6), and DMSO (DMG, n = 6) groups. Only a lung biopsy was performed in CG. In the experimental groups, blunt thoracic trauma was induced by dropping a cylindrical metal weight (0.5 kg) through a stainless steel tube onto the right hemithorax from a height of 0.4 m (E = 1.96 J). In the SG, 1 mL of physiologic saline was injected intraperitoneally, in the DXG 10 mg/kg of DX was injected intraperitoneally, and in the DMG 1.2 g/mL of DMSO was injected intraperitoneally 15 minutes after trauma. After 6 hours, lung biopsy was performed for histopathologic and oxidative injury markers.
Results Histopathologically, congestion, hemorrhage, neutrophil infiltration, endothelial-nitric oxide synthase (E-NoS), and total pathologic score were significantly higher in SG, DXG, and DMG when compared with CG (p < 0.05). Neutrophil infiltration, total pathologic score, and E-NoS were significantly decreased in DMG when compared with SG and DXG (p < 0.05). Biochemically, superoxide dismutase (SOD) level was significantly higher in SG, DXG, and DMG than in CG. SOD level was significantly lower in DXG and DMG than in SG (p < 0.05).
Conclusion DMSO prevents further injury by decreasing neutrophil infiltration and endothelial injury in lung contusions. DX may have a role in the progression of inflammation but not in preventing the pathologic disruption of pulmonary parenchyma.
This manuscript was presented at The 30th National Congress of Turkish Pediatric Surgeons and was awarded with The Best Investigation Award of Prof. Dr. Ihsan Numanoglu.
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